Identification And Analysis Of QTL Controlling Seed Tocopherol Content In The Genome Of Brassica Napus L.

Vitamin E is one of the important micronutrient,it comprises a distinctive group of potent antioxidant compounds,and it is also called tocopherol.As its strong anti-oxidation,it becomes an essential element of animals and plants.Rapeseed is one of oil seed which are rich in vitamin E.Rapeseed oil as an edible oil is very population in China,in addition,its extensive employment in European's transportation,which calls scientists to improving the quality of oil,and increasing vitamin E content in the rapeseed develops one scientific research direction.The winter-typed B.napus variety Tapidor and the semi-winter typed variety Ningyou7 are two parents.A DH population named with TN DH population was constructed with the two varieties,and a RC-F₂ population was constructed based on TN DH population in our lab.TN DH population was planted in three environments during two years,while RC-F₂ population was co-grew with the TN DH population under the environment in Jingzhou.Considering these factors influencing synthesis of vitamin E in rape seed,we improved the measurement firstly,taking the volatilization of hexane into account.Secondly,we found that different tocopherol composition was influenced by environment factor,α-tocopherol andγ-tocopherol showed much more sensitive to environment factor than total tocopherol did.Under the same environment,RC-F₂ population performed better than TN DH population about tocopherol content in the seed. This study also analysed two Brassica germplast population's vitamin E content,α-tocopherol content detected in Brassica rapa was much less than in Brassica napus.Combined TN map information,with QTL analysis software WinQTLcart2.5, QTLmapper2.0,there were 54 QTL(p=0.05) detected with the two populations.After integration with BioMercator-2.1 software,there still were 25 QTL,and 5 interaction loci pairs were detected.Asα-tocopherol has high activity,the QTL controllingα-tocopherol content—QtcA07-1 was selected,according to the information of QtcA07-1,BC₄F₂ was built up.With BC₄F₂,QtcA07-1 was confirmed,and the confidence interval was shortened,and the contribution from QtcA07-1 was enhanced.At the same time,Hpt1 enzyme belongs to the metabolism pathway of tocopherol,it was designed as a marker, and have been located in QtcA07-1.However,the result did not bring any more change except shortened QTL confident interval.