Studying On Morphology Of E.perekropvi And The Establishment Of PCR For E.perekropvi

Actinobacillus Pleuropneumoniae is the causative agent of Porcine Pleuropneumoniae, a worldwide disease causing tremendous economic loss to the swine industry. It is a common infectious disease which infects the respiratory tract of pigs. The symptoms of infected pigs induced depression, difficult respiratory and pneumonia. With the development of the raising cosmically, Porcine Pleuropneumoniae becomes one of the five primary disease that threaten mordern pig industry.The following two parts were included in the studies:Part I: The isolation of Actinobacillus PleuropneumoniaeThe bacterias isolated from zibo area, were identified by bio-chemistry experiments,PCRand injected the mouse .The results of coloration showed that 20 strains were small bacilus ,the results of bio-chemistry experiments and PCR reveled that these 20 strains were Actinobacillus Pleuropneumoniae. According to morphology observation, culture characteristic, biochemical characteristic, PCR and animal test, the disease breaking out in zi bo aera was Porcine infectious pleuropneumonia. Serotypes of 20 strains were identified,the results showed that 13 strains are serotype 5 and the left are serotype 7, serotype 5 was the dominating serotype. Part II The production of bivalent inactivated vaccine of Actinobacillus Pleuropneaumoniae and the protection test of mouse mmunity.One vaccine out of serotype 7 and serotype 5 was made basing on the epidemic of APP in zi bo aera. And then, the produced vaccine were used as antigens to challenge the mouse with the same serotype bacteria, the results showed that the mouse were well protected immunized with 0.6ml vaccine . The mouse challenged with the same serotype bacteria were not dead at this dose , while the mouse of control group were all dead. Two days after immunization, antibody was examined in the mouse. At the 6th day, the mouse exhibited significantly high antibody against APR Then the antibody slowly began to decline.Different vaccine were used to challenge the pig with the same serotype bacteria, the results revealed that the pig of control group behave clearly symptom,and there were two pigs dead. The pig which immunitied the bought vaccine behave lightly symptom,while the pig whih were injected the vaccine produced by our were well protected. These results revealed that the vaccine produced by ourself were usefull in defending APR...