The Subcellular Localization Of Centrosome Duplication Protein

Embryo adoption of modern bio-engineering technology is to enable livestock to the high-yield,high efficiency,high-quality,intensive development of an effective way.However,in vitro fertilization and embryo cloning and embryo development constraints is the low rate of these two techniques to produce the greatest obstacle to practice.This study by immune staining methods,from the Centre copy of the key regulatory genes start CDK2 and P53,and P53 on CDK2 in the 18-26 hours of in vitro mature cattle mature oocytes in the subcellular localization,and reveal them in the centre Copy of the process of location,of CDK2 and P53 in cattle mature oocytes of expression,from the centre of abnormal copy clarify the perspective of in vitro fertilization and embryo cloning and embryo chromosome instability.The results are as follows:Study of the mammalian embryo immune staining conditions and steps.Through the fertilized eggs of mice CDK2,P53 andγ-tubulin protein the immune staining on the immune staining the conditions and steps to continuously optimize,modify, simplify and development,worked out a comprehensive view of the immune oocytes Staining steps.1 cells in mouse embryos G1 phase,through a fixed,closed,dyeing,in the fluorescent microscope observation,found that CDK2,P53 andγ-tubulin protein were found positive.CDK2 and P53 in cell mitosis G1 phase of positioning at the centre,located in the nucleus area.Training were 18 h,22h,26h after the cattle mature oocytes,with the same test a fixed,closed,dyeing,in the fluorescent microscope observed that(1) 18 h after the train cattle mature oocytes,CDK2,P53 andγ-tubulin protein were found negative.(2) 22 h after the train cattle mature oocytes,the majority of oocytes CDK2,P53 andγ-tubulin protein were negative in only a small number of oocytes CDK2,P53 andγ-tubulin protein appears positive staining.(3) 26 h after the train cattle mature oocytes,the majority of oocytes CDK2,P53 andγ-tubulin protein were negative in only a small number of oocytes CDK2,P53 andγ-tubulin protein appears positive staining.(4) were positive in the event of the cattle mature oocytes,CDK2 and P53 protein location at the centre of distribution in the nuclear area.CDK2 and P53 protein is the central body and cell reproduction of the two closely related proteins,their function and a normal cell development of the split.In vitro embryos for cattle production in cattle oocytes,CDK2 and P53 protein expression of the normal or not,is a direct impact on the efficiency of cattle production in vitro embryonic one of the reasons.