Polymorphism And Genetic Effect Of FSHβ And FSHR Gene On Litter Size Of Small Tailed Han Sheep

In this paper single nucleotide polymorphisms of follicle-stimulating hormone beta-subunit (FSHβ) gene at CDS regions (exon2 and exon3) and FSHR gene at exon10 were detected by PCR-SSCP in Small Tailed Han Sheep, Tibetan Sheep,Gansu Alpine Merino, Texel sheep and crossbred of Poll Dorset and Mongolian, genetic polymorphisms of FSHβgene at CDS regions and FSHR gene at exon10 in different sheep populations were explored and its genetic effect on litter size were analyzed in Small Tailed Han Sheep. These results will provide effective molecular marker for selectively breeding about high prolificacy in sheep. The results are as follows:①Genetic polymorphisms of FSHβgene at exon2 in 5 sheep populations had been detected. But FSHβgene at exon3 and FSHR gene at exon10 had no polymorphism.②There was one single nucleotide mutation (T147→C147) at exon 2 of FSHβin the BB genotype in comparison to the AA genotype, but this mutation did not cause amino acid change.③Genotype distribution had some differences between five populations. The results of Hardy-Weinberg equilibrium test indicated that the studied populations except Texel reached equilibrium (P>0.05). All of the populations had intermediate polymorphism information contents (0.2570), comparing results of construction with evolution results in animal kingdom revealed that related species in taxonomy were respectively grouped together.⑥Sequences of FSHR gene at exon 10 between sheep and goat, bovine, mouse, pig and human were alignment, the results showed that homology was highest between sheep and goat, the next was bovine, pig and human, and the lowest was mouse.