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Changes Of Some Oxidization And A Reduction Enzymes Of Crop Straws During Composting At High Temperature And Static State

Posted on:2009-12-23Degree:MasterType:Thesis
Country:ChinaCandidate:L N SunFull Text:PDF
GTID:2143360272465766Subject:Biophysics
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China is a large agricultural country,each year,a large number of crop stalks were producted.Straw is a rich and green renewable resources,but it was not a good use,for a long time.As the most burning and the use of the approach not only resulted in waste of resources, but also cause serious air pollution.As a result,the effective use of straw is a very important significance in China and the world economy and healthy development.In recent years,the straw compost treatment has been widespread concern in the academic and research has become a hot spot.Oxidoreductase are involved in the process of biochemical reactions of a class of enzymes important to understand its Changes,you can accurately grasp the composting process,the process of humus and strength,in order to optimize the composting process and conditions to speed up the composting process and provide a theoretical basis.In this paper,wheat straw and corn stalks for the study,the use of urea-conditioning C/N,a study of static high-temperature composting process of catalase,polyphenol oxidase, dehydrogenase,peroxidase and other physical and chemical indicators of changes in the law, the following were the main conclusions:Two experiments was designed with wheat straws and cron straws respectively,urea was used to change C/N.There were three treatments in the experiment with wheat straws,one was with adding microorganism agent with Glucose(G+J),two was adding microorganism agent with(J),three was without adding microorganism agent(CK).The changes of cornposting temperature and oxidoreductase activities and their relationship during aerobic fermentation in high static state were studied in this paper.The results showed that:Catalase activity kept a lower activty in the early stage and increased quickly later,after that it kept stably at a higher activity for different treatments.Catalase activity of CK increased from the 6th day of composting while Catalase activity of G+J and J increased from the 4th day of composting.Catalase activity of G+J and J was higher than that of CK from the 19th day of composting to the end;The polyphenol oxidase activity in the treatment of G+J and J was bigger than that of CK during the prophase and anaphase of composting,It indexed that adding microorganism agent can reinforce lignin-degrading and transition of products;Dehydrogenase activity of J was significantly greater than that of G+J and CK.Peroxidase activity of adding microorganism fungus was higher than that of CK from the 12th day of composting to the end,it showed that adding microorganism agents could promote oxidation of raw material.Three kinds treatments of Perroxidase activity reached the peak in the middle stage of the composting,the activity of J Peroxidase was higher than G+J and CK from the 4th day of composting to the end,In the experiment with cron straws,there were two treatments in this experiment,one was with adding microorganism agent(J),another one was without adding microorganism agent(CK).The results showed that:Catalase decreased in the early stage and increased quickly later,after that it kept stably at a higher activityfrom the 12 th day of composting to the end for both treatments.Catalase activity of adding microorganism agent treatment was bigger than that of CK from the 9th day of composting to the end.The polyphenol oxidase activity in the treatment with microorganism agent was bigger than that of CK in the middle phase of composting,It indexed that adding microorganism agent can reinforce lignin-degrading and transition of products;Dehydrogenase activity of adding microorganism agents was significantly greater than that of CK from the 4 th day of composting to the end. Peroxidase activity of adding microorganism fungus was higher than that of CK from the 6th day of composting to the end,it showed that adding microorganism agents could promote oxidation of raw material.
Keywords/Search Tags:straw, static high-temperature composting, catalase activity, polyphenol oxidase activity, dehydrogenase activity, peroxodase activity
PDF Full Text Request
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