The Profile Of Gene Expression From Macrophage THP-1 Infected By Mycobacterium Tuberculosis Bovis And The Genotype Of Mycobacteria Tuberculosis Of ChangPing District Of Beijing

Bovine tuberculosis from Mycobacterium tuberculosis and other groups of Mycobacterium tuberculosis caused by a chronic consumption of transmitted diseases,and for its main performance of cattle decreased productivity,mastitis and tuberculosis pleurisy symptoms.Bovine tuberculosis in countries around the world have occurred in China is still the most common diseases,the International Veterinary Authority(OIE) will be classified as category B of its animal disease.At the same time bovine TB is a zoonotic disease,the bacteria can be inhaled aerosol or eating contaminated dairy products from infected cattle to people;In addition,people can exhale bacteria through aerosol transmission to cattle.Thus its spread and prevalence seriously impact on animal husbandry,food industry and sustainable development of human health.Accurate detection and elimination of Mycobacterium tuberculosis infection cattles is the key to control disease,it can be used for diagnosis of bovine tuberculosis immunity after the election is still one target for bovine tuberculosis prevention and control of the hot spots.1.The profile of gene expression from macrophage THP-1 infected by Mycobacteruim tuberculosisIn this study,Mycobacterium bovis(AF2212/97) and Mycobacterium tuberculosis (H37Rv)are the mode of infection macrophages(THP-1),Containing 22,000 full-length gene microarray study of bovine Mycobacterium tuberculosis bovis(AF2212/97),and Mycobacterium tuberculosis(H37Rv) led invasion of macrophages genome-wide expression after 72 hours at the transcriptional level Spectrum changes.In determining the analysis of the gene microarray by the specificity and stability are achieved on the basis of requests,through GO significant statistical analysis to study gene expression in the corresponding protein molecular functions,biological processes and cell components; further significant use of Pathway Statistical analysis of differences in protein expression on the interaction of each other.THP-1 cells which were infected with H37Rv,revealed the existence of 290 different gene expression,the expression of which are 170 up,120 down expression,and the results similar to the previous,in the analysis of the 22,000 genes in Mycobacterium tuberculosis infection in THP-1 cells in 1899(8.6%) differences in gene expression,1075 gene expression was increased(56.6%),the other was down 43.4 percent. 1,899 of 1,674 genes in GenBank recorded,263 of the new gene,MMP-12(H200000442) was raised to 60.5 times while the largest reduction in the protein serine/homocysteine inhibit enzymes(H200006177) was Down 6.29 times.It showed that these genes corresponding to the number of inflammatory factors,apoptosis proteins,extracellular matrix protein,T-cell receptor signaling pathway,and other related proteins.One of the inflammation-related protein 47,such as TNF,IL8,CXCL3,CCL8,CCL7,IFNK,IL-26, CCL28,and so on.The study of gene expression profile can apply the pathogenesis of bovine tuberculosis for diagnosis and study of biology great deal of useful information.2.The genotype of Mycobacteria Tuberculosis of Changping district of BeijingIn this study,Changping District of Beijing's 337 clinical TB strains as the experimental strain.Construction of the spacer oligonucleotide(Spoligotyping) and Real-time PCR typing method,a preliminary identification of TB identified Beijing genotype of Mycobacterium tuberculosis and non-Beijing genotype of Mycobacterium tuberculosis.At the result,299 strains of the Beijing genotype Spoligotyping map (including the Beijing genotype category),according to Beijing/W definition of Mycobacterium tuberculosis,which accounted for 88.7%of all strains(299/337).A Real-time PCR method,based on RD105 for the target of 337 Mycobacterium tuberculosis. Lack RD105 which the Beijing/W of Mycobacterium tuberculosis to 299,accounting for 88.7 percent of all strains(299/337).Spoligotyping identified the Beijing/W RD105 identification with the Department of the Beijing/W in the same results.RD181 strain of atypical Beijing is 47(15.7 percent),while the relative lack of modern Beijing RD181 are the typical family strains were 252(84.3%).In a typical family of strains in Beijing,RD150 and RD142 missing strains were 168 and 173.Use two-way system at the Beijing genotype pedigree and typical and atypical Beijing genotype results of the definition of the basic agreement(the same rate of 99.09%),but the new method of typing is faster,more convenient operation to prove that this new The feasibility,accuracy and convenience.