Study Of The Degradation Of Lignin By Microbes In The Compost Of Agricultural Waste

It is a current trend to degrade agriculture waste in composting process using different microorganism. The species (composting), biomass (richness) and the community (structure) of microorganism are dominating factors in composting. The traditional cultivating method is falling into disuse since its high deviation. However, the quinine profile analysis can overcome which based on the different kind of isoprenoid quinine in microorganism. This paper illuminated the establishment of the quinine analysis system and successfully using it to characterize the microbial community in compost. The microbial community having the potential to degrade lignin was also investgated. The major quinine species in compost process were MK-7(H4), Q7 and Q8, indicating thatγ-subclass Proteobacteria and actinomycetes are the major microorganism. The results of community changing in different compost period that obtained through the quinine ananlysis accorded with that obtained through traditional cultivation. In the community having the potential of degrading lignin, MK-7(H2), MK-7(H4) and Q9(H2) were found to be the major quinines, which mostly represent some fungi and Proteobacteria. The microbes worked mostly in the high-temperature period and the second fermenting period. Both of the two microbial communities had relatively small diversity of quinine (DQ), which indicated the singleness of the microbial species.The effects of biosurfactant rhamnolipid on the enzyme production by Phanerochaete chrysosporium and Penicillium simplicissimum were also studied. Results show that the effects were connected with the concentration of rhamnolipid, adding ways, fungi species and type of enzymes. Rhamnolipid at 0.0065% increased the activities of LiP, MnP by P. chrysosporium, LiP, laccase by P. simplicissimum, while rhamnolipid at 0.013% only stimulated the production of LiP by P. chrysosporium, laccase by P. simplicissimum, but restrained the other two enzymes'activities. However, rhamnolipid only made the highest MnP of P. simplicissimum coming ahead of which of the control, with no change of MnP activity. All the degradation rate of lignin which with rhamnolipid was higher than that of the control, and inoculation of Pseudomonas aeruginosa showed the same or even better stimulative effect comparing with those rhamnolipid was added.