| The reduction of cereals induced by flood every year in our country especially in southern lowland is rather serious. Flood will retreat after a long time, which results in yield reduction or even death of rice (Oryza sativa L.), a kind of crop mainly planted in south China. Therefore, understanding of the submergence-tolerant mechanism of rice can help people to find a way to improve the submergence tolerance of rice and consequently increase the yield of rice.This study used the leaves of submergence-tolerant rice variety FR13A as experimental materials. FR13A was firstly treated with complete submergence at tillering stage and then its leaves completely submerged for 0 h, 2 h, 4 h, 8 h, 16 h, 32 h were respectively sampled to execute mRNA differential display. Eight cDNA fragments were isolated by mRNA differentially display reverse transcription PCR (DDRT-PCR), but only four fragments were approved truth by reverse Northern dot blotting. These fragments named as DF8, DF9, DF10, DF11 were sequenced and then analyzed by Blast program. The putative protein of DF8 was highly homologous with WD-40 repeat protein and that of DF11 was homologous with auxin responsive GH3-like protein. The two rests did not found homologous proteins.Due to sampled at early stage of submergence, these cDNA clones may be some regulative genes participated in signal transductions from outside to inside of cells and then induced the expressions of related genes. |
