| As progressing on postharvest induced resistance in muskmelon, it is necessary to analysis the expression of defense genes in treated fruit. In this paper, RNA was extracted from muskmelon"Yindi"(Cucumis melo L. cv. Yindi)after screening on major factors of Trizol method. The expression of seven defense genes was analyzed by RT-PCR in fruit treated with sodium silicate at 100mmol and challenged with Trichothecium roseum. The results indicated as below:1. The most amount of extracted RNA was in 200mg sample with 1ml Trizol. A260/A280 of RNA was beween1.9 and 2.0 in the condition of chloroform extractived twice. The extracted RNA was not polluted by protein and DNA and was transcribed reversely. Both room and low temperature sediment had no singinfigant effect on RNA yield. Amplified geneβ-actin was same as designed after the production of reverse transcription diluted five times. It was proved that extracted RNA could be used for RT-PCR .2. The cDNA template quantity was homogeneity after adjusted several times. The gene of MCHT, PAL, SOD, POD, APX and LOX was verificated by standard Marker method. CHS was verificated with test sequence analysis method and the sequence in muskmelon was gotten. The results showed that the target genes could be vertificated by standard Marker method and the test sequence analysis method and the designed primers could be used for expression analysis.3. The melon treated with sodium silicate and the challenge with T. roseum advanced MCHT and SOD genes expression maximum for 2 days, and the expression of treatment was more than the control. The treatment and challenge improved PAL and CHS genes expression, prolonged expression maximum of POD genes for 2 days, and increased POD gene expression after treatment and challenge conditions. However, APX and LOX genes expression were inhibited by the treatment. The results suggest that fruits resistance is induced by treatment of sodium silicate.
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