Immunological Activity Of Linear B-cell Epitopes On CSFV E2

Three recombinant prokaryotic vectors, pTA, pTB and pTC were constructed containing classical swine fever virus C strain E2 linear B-cell epitope gene(A/D antigenic domain and B/C antigenic domain) using pET 32a(+) expressing vectors. Their expressing characteristics were compared and it was found that pTA, pTB and pTC in BL21(DE3) can present high production of recombinant protein. Then, expression of protein were optimized by inducing in different time and different concentration of IPTG. After that, the recombinant protein was purified by Ni-NTA affinity chromatography. Purified protein was quantified and detected by SDS-PAGE and Western-blot. The purified proteins mixing with Freud's adjust and inoculated to porcines. After vaccination, specificity antibody titers against CSFV and relative cytokines production were detected by ELISA.