The Separation And Identification Of Pathogenic Microorganism And Drug Selection Of Hyriopsis Cumingii

Hyriopsis cumingii is one of the main freshwater pear meussel scattering kinds of our country raised in fresh water.It is widely covered the Eastern,the Southern parts and central section.In this study,gaining the healthy and plague diseased mussels of Hriopsis cumingii lea from Changde city of Hunan province to find the resource of disease,and select the useful drug to control and prevent epidemic diseases of Ityriopsis cumingii.Clutivate excellent germplasm resources,so as to improve output of freshwater pear.The separation and identification of bacteria of Hyriopsis cumingii and determined pathogen:obtained diseased material from different tissues of diseased Hyriopsis cumingii.Separated,purified and identificated the pathogenic microorganism,we got 6 kinds of bacteria,seudomonas alcaligenes,Aeromonas sobria,Serratia marcescens,etc.and determined Aeromonas sobria and seudomonas alcaligenes as pathogen that caused Hyriopsis cumingii disease.The selection of Chinses herbs and antibiotics:By way of paper diffuseion to determine the antibacterial diameter and microdilution method to determine the Minimum Inhibitory Concentration(MIC) and the Minimum Bactedacidal Concentration(MBC).Studied the antibacterial activity of 30 kinds of Chinses herbs and 20 kinds of antibiotics.the result was,at the concentration of 1g/ml,the antibacterial diameter of Rhicoma Coptidis on Aeromonas sobria and seudomonas alcaligenes are 20.13mm,16.29mm,respectively,the MIC were 0.0125g/ml and 0.0104 g/ml,respectively,the MBC were 0.0125g/ml and 0.0208g/ml,respectively; the corresponding results of Fructus Mume were 18.26mm and 20.38mm, respectively,the MIC were 0.0125g/m and 0.0104g/ml,respectively,the MBC were 0.0208g/m and 0.0167g/ml,respectively.Ciprofloxacin and Levofloxacin had the higher antibacterial activity in the 18 kinds of antibiotics,the antibacterial diameter of Ciprofloxacin on Aeromonas sobria and seudomonas alcaligenes were 25.64mm and 25.24mm,respectively,the MIC were 1.667μg/ml,1.667μg/ml,respectively,the MBC were 2.083μg/ml,2.083μg/m,respectively,the corresponding results of Levofloxacinare25.48mm and 24.67mm,respectively,the MICare 1.667μg/ml andl.250μg/ml,respectively,the MBC were 2.917μg/ml,2.500μg/ml,respectively.By way of orthogonal experiment to determined combination:combinate Lomef loxacin,Levofloxacin,Rhicoma Coptidis and Fructus Mume that selected before, according to L₉(3⁴) to design orthogonal experiment,we descovered that the best combination to Aeromonas sobria were 0.5 MIC(0.005g/ml)of Rhicoma Coptidis,1MIC(0.01g/ml) of Fructus Mume,1MIC(1.667μg/ml) of Iprofloxacin And 1MIC(1.667μg/ml) Levofloxacin,the best combination to seudomonas alcaligenes were 0.5 MIC(0.005g/ml) of Rhicoma Coptidis,1MIC(0.01g/ml) of Fructus Mume, 1MIC(1.25μg/ml) of Ievofloxacin And 1MIC(1.0μg/ml) Iprofloxacin.Iprofloxacin and levofloxacin were key factors that effected the antibacterial activity.