Studies On Extraction, Isolation And Purification Of Chrologenic Acid In Lonicera Macran Thoides Hand.-Mazz.

Lonicera macran thoides Hand.-Mazz,is a regional produce as Flos Lonicera's commodity drug in Hunan province.Flos Lonicerae,according to clinical effects and regional habit of using medicine in CP-2005,comes from the dried flower bud or newly-burst flower of Lonicera macran thoides Hand.-Mazz,L.hypoglauca Miq.,L.confusa DC..With a great pharmacological effort of anti-pyrexia,detoxification,Because of its unique efforts of curing carbuncle, turgescence,furuncle,laryngic numbness,erysipelas,hot toxin,rheum luke warm fever and so on.Chlorogenic acid is one of the active constituents from Lonicera macran thoides Hand.-Mazz.As a major source of chrologenic acid in china,much higher than the traditional Flos Lonicerae japonicae in the content of chrologenic acid.There is a little reports of Lonicera macran thoides Hand.-Mazz.in china.Especially the purification and isolation techniques and pharmacology functions of chrologenic acid.That situation will greatly baffle the research and development of natural medicine and functional food based on the material of Lonicera macran thoides Hand.-Mazz.with the continuous deeply development of international interaction. Lonicera macran thoides Hand.-Mazz.will be becoming the major resources in the international markets.This project aim at extraction,isolation,purification of chrologenic acid by modern technologies.The objective of present study is to offer a reliable theory basis for people to make full use of chrologenic acid in Lonicera macran thoides Hand.-Mazz..1 Study on the examine methods of chrologenic acid compounds(1) ultraviolet spectro photometryConsult on the standard of chrologenic acid,Full wavelength scan,ensure the upmost absorb peak in 327nm of chrologenic acid standard sample and chrologenic acid sample,the standard curve,the regression equation was y=0.0198x+0.0005,r=0.9993,the favorable linear relationship between solution concentration and absorbency was at the rang from 0.002～0.01 mg/mL.(2) High performance liquid chromatography(HPLC)HPLC was introduced to the concentration and content measure of chrologenic acid substance, measure condition: Chromatographic instrument:LC-10AT_VP HPLC(shimadzu japan)Chromatobar:chromatobar(Kromasil 200 mm×4.6 mm 5μm)Mobile phase:acetonitrile-1%acetum(13:87)Inspection wavelength:327nmSample quantity:10μlFlowing speed:1mL/minColumn temperature:35℃2 Study on the extract processing of chrologenic acid compounds in Lonicera macran thoides Hand.-MazzOptimized extract technics parameter of chrologenic acid compounds in Lonicera macran thoides Hand.-Mazz.Ensured the upmost extract condition:70%ethanol solution(pH 5.0),the ratio of material and alcohol was 1:25(w/v),extract temperature 70℃for 60 minutes twices.The chrologenic acid content of the extract products reached to 6.55%.3 Study on the purification and isolation processing of chrologenic acid compounds in Lonicera macran thoides Hand.-MazzThe purification and isolation processing of chrologenic acid compounds adopted the macroporous resin.By filtrating kinds of different macroporous resin,though static adsorption and dynamic adsorption experiment.The result as follow:The macroporous resin NKA-9's absorption content of chrologenic acid compounds is 27.78 mg/ml,the desorption ratio reached to 85.3%.dynamic adsorption content is 27.55mg/ml.The products(pure degree)is 75.68%after purification though macro porous resin.the reclaim ratio reached to 82.38%.