The Diversity And Analysis Of Bacteria In Liaoning Bay And Aquaculture Ponds Of Sea Cucumber

Content:The diversity of bacteria in Summer sea water of Dalian Bay and Liaodong Bay and the Autumn sea water of Liaodong Bay and aquaculture pond of sea cucumber were analyzed by constructing 16S rRNA libraries of bacteria samples collected from four Survey Stations, this will provide helpful information for investigating and empoldering microbial resource and sea cucumber cultured. The results showed that there were mainly uncultured bacteria in the four Stations. In Dalian Bay there were 51 unique sequences belonging to 13 bacteria species (genera), in Liaodong Bay (summer) there were 53 unique sequences belonging to 13 bacteria species (genera), in Liaodong Bay (autumn) there were 37 unique sequences belonging to 10 bacteria species (genera) and in aquaculture pond there were 39 unique sequences belonging to 12 bacteria species (genera). Different preponderant bacteria existed in the different stations, no pathogenic bacterial species were found in Dalian Bay and Liaodong Bay stations. This indicated that the bacterial diversity exists in the two stations. We found Vibrio.alginolyticus, Brachybacterium qaraconglomeratum,Bacillus megaterium and Nesterenkonia sp. in the aquaculture pond, so we should control it and reduce baleful bacteria, this will provide helpful information for the cultured of sea cucumber.The strains were studies using a polyphasic approach, which included bacillus culture character observation, biochemical characterization and phylogenetic analyses by direct sequencing of 16S rRNA genes, et al. The associated with dominance heterotrophic bacillus composing on accrete media of different periods in cultured Apostichopus japonicus and disease revealed that the dominance strains which were isolated continuously three times from the year 2005 to 2006 were identical. And the dominance strains mainly included Vibrio.alginolyticus, Brachybacterium qaraconglomeratum, Nesterenkonia sp., Bacillus megaterium. Manual infection tests showed that bacillus could cause different degree disease. Drugs sensitivity tests showed that these dominance bacillus were sensitive to Nitrofurantoin,Neomycinum and Cefamandde.