| Alfalfa (Medicago sativa L.) is the world's most widely distributed perennial legume forage grass, and also is the most important one of the legumes forage grass. It is not only high yield but also nutrient-rich, so it is called the"king of grass". Traditional breeding methods is mainly through the hybridization to abtain the new varieties. It required long cycle and heavy workload. Using molecular biology methods is the hot spot of current breeding research, especially applied to the resistance breeding work, it can shorten the breeding period, improve breeding efficiency.This study is mainly about a zinc finger protein gene'promoter. Zinc finger protein is the most rich protein in a class of transcription factors in eukaryotic genome, such proteins binding the Zn2 + to form a stable finger structure, it play an important role in gene expression regulation, cell differentiation, embryonic development,eta.The function of zinc finger proteins in the animal and human body have been penetratelly researched, for the past few years, through constantly investage, we have find the zinc finger protein also playing important role in plant.participated most kinds of resistance reaction in the plant progress of growth.Promoter is the originate sequence of genetic transcription,it can discrimination and bind to the RNA polymerase.As an important regulatory element, the research of promoter has became the hot point of molecule biology. To study and research the function of promoter, having the important meaning for us to understand the growth and development growth,investage the fit mechanism of the biology and it habitat,controlling the expression of exogenous in the transgenic plant.The principle consequence of this research is just as follow:1. Using the nested PCR, obtaining the MsZFN gene's promoter sequence,about 1500bp, to compare the homology in the blast database,this gene is obtained for the first time, it has 88% similiraty with the Medicago truncatula chromosome 7 clone mth2-36p20, Logging in the Genebank, accession number is FJ161979.1,named MsZPP, To analysis the sequence, found many CAAT-boxes and TATA-boxes, TATA-box can lead the RNA polymeraseâ…¡to the right transcriptional start site, CAAT-box mainly control the starting frequence of transcription, is an element of regulate transcription starting,having no direct relationship for the specification of the promoter. It also found some potentional cis-acting element, for example, the ABRE ,which is related to the ABA derivation; the MBS,which is related to drought induction; the TGACG-motif, which is related to the jasmonic methylester,and some photoresponse elemant, such as ACE,G-box,GT1-motif, I-box, SpI.2. constructing the complete sequence expression vector and translating tobacco, obtaining the transgenic plant, PCR detection shows the objective gene has intergrated to the tobacco genome.3. According to the transcriptional start site which has been predicted, cloning the different sequence which is short of the different length of 5'sequence.Constructed the expression vector, translated tobacco, obtained the transgenic plant, PCR detection shows the objective gene has intergrated to the tobacco genome...
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