The Effection Of Interferon-γ To The Immune Function Of Rat Intestinal Mucosa Microvascular Endothelial Cells

This test isolate and culture the Immune Mechanism of Rat Itestinal Mucosa Microvascullar Endothelial Cells(RIMMVECs) successfully in vitro, in order to study the effection of IFN-γto RIMMVECs'immune mechanism, stimulating the cells with different concentration IFN-γ,using flow cytometry to test the expression of ICAM-1 which is an important immune-related factor, while testing the quantity of IFN-γR which express on the cells'surface; Using the ELISA kit to test the expressing of IFN-α/βin cell culture supernatant. Results: RIMMVECs express IFN-γR fundamentally, the expressing of ICAM-1 increased greatly in both different concentration group at 6h, IFN-γdid a little effection to the expression of IFN-α/β. Conclusion: The combination of IFN-γand IFN-γR could accelerate the excretion function of RIMMVECs, up-regulation the expessiong of ICAM-1, IFN-γcould participat the RIMMVECs'immunoreaction and inflammatory reaction while play an important role on correspondent regulation.