Study On DNA Fragment Damage Of Gypsy Moth's Egg Treated By Gamma Ray

Gamma ray exposure deinsectization is one of effective methods in the current many physical preventing and controlling technology.Research the damage mechanism of gamma ray exposure deinsectization,explore the relationship between insect egg cell radiation biology effect caused by gamma ray radiation and radiation dose,seek the suitable radiation dose,can provide theoretical data for the research and the practical application of gamma ray exposure deinsectization.This research use the different dose gamma ray exposure gypsy moth egg;use the molecular biology technology and the cytobiology technology and some other biology technology in studying the mechanism of the gypsy moth egg's cell damage caused by gamma ray radiation.The major damage the gamma ray exposure to the gypsy moth egg is to its chromosome damage.Three methods were introduced in the experiments to research biological effects of radiation caused by different doses of gamma ray radiation.One is the DNA gel electrophoresis,the other is AO super vital staining.The research indicated that,the gypsy moth egg hatchability obviously reduces with the increasing gamma ray radiation dose.The gypsy moth egg hatchability and hatching rate and DNA damage degree and programmed cell death have obvious effect relations of dose with the gamma ray radiation dose.Gamma ray could break gypsy moth egg's DNA to become smaller DNA fragments.Increases with the gamma ray radiation dose,the chromosome fragments become smaller,cell chromosome are seriously damaged,thus causes the cell division is delayed,even some cells stop splitting,until death.After gamma ray radiation,the gypsy moth egg cell chromosome extent of damage and the cell survival percentage are all good relation with the egg hatching rate and hatching progress.250Gy gamma radiation could speed up the development of gypsy moth egg,but did not cause higher hatching rate.400Gy Gamma radiation could be a good dose of deinsectization,because of egg hatchability lower than standard of quarantine,both may guarantee the deinsectization-effect and save energy sources.Suitable radiation dose of gamma ray can be an effective method for pest controlling.It provides theoretical data for the physical deinsectization.It also provides an effective method for pest controlling,food exposure processing,the import and export of agricultural products. After thorough research,it will certainly to become an important method for pest controlling in many realms of production and life.