Analysis On The Transgenic Populus Davidiana×P.bolleana With Genes Concerning Resistance To Adverse Condition

This research,using A tumefaciens mediated method,transformed the lea(late embryogenesis abundant) into genome of Populus davidiana×P.bolleana.Eighteen buds that were resistant to kanamycin were obtained.After detecting them with PCR,the results showed that these buds were positive.And then the total RNA was extracted for RT-PCR to confirm that the exogenous gene had expressed on RNA level.These transgenic plants were treated with 0.8%NaCl.And then the content of malondialdehyde and chloroplast were measured besides activity measurement of other four anti-oxidative enzymes including superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)。The results indicated that on the fourth and sixth day of treatment,the average content of chloroplast in transgenic plants were higher than control,14.28%and 16.42% respectively.Judging from the activity fluctuation of four anti-oxidative enzymes in these transgenic plants:(1) During the process of fourth and sixth day's stress,the activity of APX reached the peak, especially the activity of APX in the L15 and A11 were remarkably higher than control, 63.09%and 48.85%,respectively.(2)On the fourth and sixth day of stress,activity of POD in these transgenic plants(L10,P6, P8) were 37.34%,28.39%,28.99%stronger than control while that of POD in those ones were 38.6%,19.48%,19.34%higher than control.(3)On the sixth day of stress,activity of SOD in L15,P1,P8 and P8 were significantly 26.36%,27.31%,17.33%higher than control respectively.(4) On the second day of stress,activiy of CAT in all the transgenic plants except for A18 all strikingly higher than control but at the late stage of stress only the activity of CAT in L15 was 65.09%higher than control while that in L14 was higher than control.After using membership function comprehensively to analyze four anti-oxidative enzymes as well as content of chloroplast and malondialdehyde,the thorough appraise was made about the tolerance to salinity.The sequence of resistant ability from high to low is; L10>A11>P1>L14>A18>P8>P6>A16>P7.