The Study Of Applying A Dried Bacillus Subtilis Culture To Gosling Diet

This stud y was carried out to investiga te the effects of a dried Bacillus subtilisculture on gosling early performa nce．Ninety six goslings were ra ndomly assignedto 4 dieta ry trea tmen ts:(1) nega tive control (NC),basa l diet；(2) positive contro l(PC),basa l diet + Fla vomycin 120g/ton diet；(3) low dose group (Experimen tⅠ),basa l diet +Dried Bacillus subtilis Culture 250g/ton diet；(4)high dose group(Experimen tⅡ),basa l diet+ Dried Bacillus subtilis Culture 500g/ton diet,each ofwhich were assigned to 4 pens (6 birds in each of 4 pens)．Growth tra its (ABW,ADG,ADFI and F/G) were obta ined through 28d．At d 26～28,the excreta perpen were collected for determina ting dry ma tter and energy retention by innerind ica tor method (4N acid insoluble ash,4NAIA)．At d 28, segmen ts ofduodenum,je junum,ileum and ceca were removed to measure intestina lmorpholog y,and the inclusion of them were collected to determina te theammoniaca l nitrogen and vola tility fatty acid concentra tion．Lastly,we isola tedEscher ichia coli from a sa mple of 4 wk dia rrhea gosling's intestina l inclusion,the na inhibiting test in vitro was investiga ted anta gonistic activity of a dried Bacillussubtilis culture aga inst the Escher ichia coli．The results showed:1. Avera ge bod y weights of Experimen tⅠat d 21 and 28 were increased by9.2% and 6.85% compa red with those of NC (P＜0.05 ),but had no significa ntlydifference from PC (P＞0.05 ),ABW of Experimen tⅠhad significa ntly differencefrom Experimen tⅡat all days except d 21 (P＜0.05 )；The ABW of Experimen tⅡdid not improve significa ntly compa red with NC (P＞0.05 ),and was the sa mecompa red with PC at all days except d 14 (P＞0.05 )．Experimen tⅠobta ined a lower ADFI tha n PC and NC (P＜0.05) during d 1～28．Morever,Experimen tⅠhada lower F/G (P＜0.05),but Experimen tⅡ(P＞0.05 ) tha n NC；Experimen tⅠred uced significa ntly F/G tha n PC and NC on d 0～7,1～14 and 1～28 (P＜0.01)．2. Retention ra te of DM and energy in 4 dieta ry trea tmen ts are 65.79%,68.04%,67.38%,68.96%,and 72.4%,74.55%,74.54%,74.21% respectively,the retention ra te of DM and energy of NC is lower tha n the others (P＜0.05)．3. Experimen tⅠincreased villus hight and V/C of duodenum and red ucedcrypt depth compa red with NC and Experimen tⅡ(P＜0.01),also increased villushight and V/C of duodenum (P＜0.01) and red uced crypt depth (P＜0.05)compa red with PC. Experimen tⅠdecreaded crypt depth of je junum (P＜0.05) andExperimen tⅡimproved villus hight and V/C of je junum (P＜0.01) compa red withNC；Experimen tⅠincreased V/C and red uced crypt depth of ileum (P＜0.01)compa red with NC,also increased V/C and red uced recess depth of ileum (P＜0.05) compa red with PC．4. ExperimentⅠred uced ammoniaca l nitrogen concentra tion of caecum andileum inclusion compa red with NC and PC (P＜0.05) but Experimen tⅡ(P＞0.05 )．5. ExperimentⅠincreased acetic acid concentra tion of caecum compa red withNC and PC (P＜0.05) but Experimen tⅡ(P＞0.05 )．6. By a bacteriosta tic test in vitro,the Bacillus subtilis showed anta gonisticactivity aga inst Escher ichia coli isola ted from a sa mple of 4 wk dia rrheagosling's intestina l inclusion when cultured Bacillus subtilis together withEscher ichia coli．In conclude,1) the diet conta ining a dried B. subtilis culture at 250 g/ton offered big benefits to growth performa nce of goslings,and these benefits couldequa l or exceed those from Fla vomycin；2) these effectua l performa nce of a driedB. subtilis culture might be rela ted with improving nutrien t retention and sma llintestine mucosa morpholog y, inhibiting intestina l ha rmful bacteria；3) it issuggested tha t the dried Bacillus subtilis culture could substitute for Fla vomycin inprod ucting Green gosling．...