Identification And Expression In H Protein Of Peste Des Petits Ruminants Virus (PPRV)

In this paper,a primer was designed and synthesized based on the sequence of the nucleocapsid(H)protein genes of peste des petits ruminants virus(PPRV),which is added BamH I sites for amplifiting the Full-length fragment of the H gene from recombinant plasmids named p TOP-H using PCR.The fragment was cloned into pGM-T vector and transformed into E.coli TOP10,DNA sequence was determined.Then the cloned fragment was subcloned into prokaryotic expression vector pET-32a(+),and the recombinant expression plasmids were expressed in E.coli BL21(DE3) with inducement.The size of the recombinant fusion protein was 80ku(H) analysised by SDS-PAGE.The western-blotting results indicated that the expressed protein can be recognized by the PPRV positive serum.