| Protein is a necessary for honey bees to keep normal growth, development and reproduction. The short supply or poor quality of protein will induce delayed development and short life span for the larvae and young workers, even that the queen ceases to lay egg, as a result, the colony will collapse. Artificial feeding is an important measure in scientific beekeeping. Artificial pollen substitutes are important protein supplement. In beekeeping , the artificial pollen substitutes are appropriate supplementary to promote and improve the breeding colony of royal jelly production when the colonies have insufficient pollen supply. Though the artificial pollen substitutes have long been prepared by experiences, its impacts on honeybees hasn't been explored yet.In this study, colonies were fed with artificial pollen substitutes composed of different proportions of rape pollen and soybean powder, and the effects were tested on the larval weight, the larval hemolymph protein content, and some other items. This work would enrich the honeybee nutrition physiological theory and provide a scientific basis for artificial pollen substitutes making. The results showed that, the body weight and protein content of 3-day-old worker bee larvae were no significant influenced by the ratio of rape pollen to soybean powder (P>0.05), while those of 6-day-old larvae were significant affected (P<0.05).The 16 kinds of free amino acids and the total amount of free amino acids of 6-day-old larvae had no significant (P<0.05)difference among the colonies fed with different ratios of rape pollen and soybean powder mixture. Fe, Ze, Cu, Mn and Se content of hemolymph had no difference between 6-day-old worker larvae fed with Feed No. 2 (75% of rape pollen +25% soybean powder) and Feed No.1(100% rape pollen), but significantly different (P<0.05) with Feed No.4 (25% of rape pollen +75% soybean powder) and Feed No.5 (P<0.01).
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