| The DREB (dehydration resistance element binding protein) is the stress-related transcription factor family. They exist in all kinds of plants, and regulate the expression of many stress-related genes. DREB transcription factor plays a role in plant resistance. DREB genes in the current study focused on functional analysis, transcription level of their understanding of less regulation and control mechanisms. In order to analyze transcriptional regulation molecule mechanism of GmDREB3 gene, the provious work in our lab indicated that some key up-regulated motif between -705~-1117 bp and down-regulated motif between -1117~-1464 bp. To identify the key cis-element, in these two sections from the 5′to 3′end deletion studies carried out paragraph by paragraph: transcriptional activation domain 1117-1, 1117-2, inhibition zones 1464-1, 1464-2, 1464-3, 1464-4. GmDREB3 differcate promoter regions were inserted upstream of GUS reporter gene. Then, these vactors containging differcate promoter fragments were transferred into wheat callus using particle bombardment. Using the method of transient expression testing different length promoter activity. The results suggested that the MYB key up-regulated motif between-705~-731 bp and the MYC key down-regulated motif between -1117~-1269 bp. Using the regulatory elements important to build a bait vector. Using one-hybrid yeast technology to screening and important upstream regulatory element binding protein regulation. The GmDREB3 transcription factor already determined that the activation and the inhibition element establishes contacts 3 repetitive sequences and insert on the pHIS2.1 vactor. After antonomous activation analysis, we choosed the 50 mM 3-AT pHIS2.1-1117-2 and 5 mM 3-AT pHIS2.1-1464-3 of the GmDREB3 as bait fragment for screening cDNA library. The construction and screening soybean cDNA library were in progress.Wheat disease is one of the main factors that affect wheat production. Breeding of disease-resistant wheat varieties is the most economical and effective method to control wheat diseases. Normally, the resistance controlled by single gene loses easily in practice. We made multiple cross by using the wheat germplasms with different disease-resistance traits or high grain quality, and developed a new wheat line Guan 4 with strong multiple disease resistances and good agronomic characteristics. Further, the genes containing for disease resistance and the quality improvement in the new line was analyzed by molecular markers. The result indicated that: Guan 4 carries resistance to both powdery mildew and stripe rust, in which the powdery mildew resistance is contributed by Pm21 from parents 92R179, while the stripe rust resistance contributed by YrX from parent YW243, The high quality genes in the new line was derived from another parent Liao 10. It was supposed that the new line would be applied in wheat breeding program widely due to its ideal HMW-GSs composition of 7+8, as well as the multi-resistance traits. Thereby, Guan 4 as excellent parent application to improve better quality and resistance to powdery mildew and stripe rust of the wheat.
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