Isolation Of Botrytis Cinerea And Extraction, Purification And Bioassay Of Toxins

BC-16 and BC-21, showed that toxins produced 2-6 bands with Rf values from 0.149 to 0.663. Among 4 isolates, respectively, had a band with similar Rf value, which maybe the active ingredient of toxins.The culture condition for the production of Botrytis cinerea toxin was studied.It was appropriate for the toxin production as follows:The suitable medium was Czapek-Dox medium.The isolates was cultured for 14-21days at 25℃with darkness and shake. SSR molecular marker was employed for genetic diversity analysis of 29 isolates except BC-25.7 SSR primers amplified 29 bands and polymorphic bands were 82.76%. The 29 isolates were clustered into three major groups with UPGMA analysis based on the SSR marker.Sporulation conditions of 13 Botrytis cinerea isolates were studied in the experiment.The results showed that sclerotia produced greater with the ABA concentration increasing. ABA has little effect on the mycelial growth rate.Spore formation medium is more conductive to the formation of spores; different kinds of media have a little impact on sclerotia.