The Biochemistry Mechanism Of The Mustardre And LFY Like-gene Cloning And Analysis

In the breeding of the mustard,we need the premature bolting to add generation propagation, shorten breeding period,and accelerated breeding process.In the seeding production of F1, regulate the parents to flowering simultaneously.Therefore,it is necessary to study the bolting and flowering of mustard.This research studied the mechanism from the physiology and the molecular two different aspects which the leaf mustard reproduction growth transforms.The experiment took the width handle mustard as a material,carried on the experiment in April,2008,after 30 days venalization,every 2 days selected physiology biochemistry targets: The soluble sugar content,POD activeness,SOD activeness and the soluble protein content,took 5 times,comparied to without venalization.Processed latter 16 days in the leaf mustard after the gibberelin,each 2 days selected physiology biochemistry targets,took 5 times,not GA processed as the comparison.The biochemistry targets determined results were as follows:The soluble sugar content was higher than the comparison level,when bolting rapid accumulated in the mustard,the max was 31mg/g,after bolting to reduce;the POD when bolting reduced rapidly,the minimumis was 11U/g.FW.min,after bolting to elevate;the SOD activeness was not account for the rule,the soluble protein content was higher than the comparison level,when bolting rapidly accumulated in the leaf mustard,then to reduce.Two kinds of different processing biochemistry targets changed tendencies were consistent.The biochemistry targets relevance analysis were as follows:Through the SPSS software analysis discovered POD and the soluble protein assumeed the inverse correlation.The leaf mustard in the process which transformed to the reproduction growth along with the soluble protein increased,the POD activeness reduced.The soluble protein and the soluble sugar present correlation,their tendency was the same,explained these two targets can suit for the breed growth transformation.SOD was not remarkable to the relevant change.LFY played important roles in floral development.As a floral meristem identity gene,LFY not only controled the transition from vegetative to inflorescence and floral meristem in plants,its overexpression can induce the plant to flower early.LFY also functioned as upstream regulator of floral organs identity genes and was a key intergrator of the outputs of floral inductive pathways to induce floral meristem transformation.We had obtained the LFY homologue in the Arabidopsis thaliana and Brassica oleracea.The sequence was 1307bp.The LFY homologue protein aligned well with the sequence of other LFY-like proteins.Some motif indicated their possible role as transcriptional activators.The LFY homologue protein was 93%identical to BOFHand 89%identical to LFY.RT-PCR experiment results showed that the LFY homologue of mustard was expressed at low levels in the leaf and young stem,but strongly expressed in flower bud,there was no expression in roots.In hybridization were carried out to localize the LFY homologue gene transcripts during flower bud development.Accumulation of LFY homologue transcripts in sepal primordial,apex of whole sepal formed and in stamen primordial.It was also expressed in mature leaf primordial and in developing leaves.