Preliminary Study On The Boron-mediated Transformation Of Soybean (Glycine Max)

Soybean (Glycine max) is one of the most important crops. It is not only a main source of vegetative protein and oil for human being due to its richness of protein , oil and other physiological chemical compositions, but has it also a wide range uses in industry and feedstuff. However, in practical soybean production there are many factors which affect its yield, such as the crop diseases, insect pests, weeds and drought. With the development of the genetic engineering technology, the transgenic approaches have been tried to overcome these problems since 1988. Actually a few of cases of transgenic soybeans were successful due to the low transformation rate. To establish an effective transformation system is still a hot spot in the soybean transformation. In this study, soybean calli are used as transgenic recipient materials to explore the new transgenic approach.At the present studies, the first step is to establish a efficient soybean callus inducing and isolated culture system, the objective inductivity of soybean callus is 100%. The second is to study the effect of boron on cell structure and shape. And the final is to transform the boron-treated soybean callus with a vector carrying GFP gene and detect the gene expression. The results show that there are some differences reaction to the stress of boron excess between cullas and plant stock. When the callus is grown in boron excess condition, the cell constitution will be changed. For example, the accretion between cells are destroyed and the volume of cells are become lager. These cells of the callus are suitably used to study the direct transformation of soybean with fluorescently-labeled carrier. The expressed green fluorescent protein (EGFP) was observed in cells using fluorescence inverted microscope.