| Hizikia fusiformis is one of the most important economic seaweeds, which has been applied in food, medical and chemical fields. In this study, we analyzed the genetic distances and constructed the fingerprint of the"Lufeng No.1"and other two good strains, which have been generally applied in the production. It was indicated that: RAPD (random amplified polymorphic DNA) was applied to analyze 125 individuals from 5 populations. A total of 135 amplification loci were obtained from 12 selected primers after screening and P% (the percentage of polymorphic loci) is 84.4%. And 4 stable and clear bands were selected to construct a fingerprint map for discrimination of the 5 populations. Among the 4 bands, one special band exclusive to"Lufeng No.1"was converted to the SCAR marker successfully. In addition, we did the genetic analysis of the 5 populations and the results showed that"Lufeng No.1"and Hizikia strain 2 can be separated with the wild population easily. Genetic distances calculated with the Dice coefficient ranged from 0.1116 to 0.2563.ISSR (inter-simple sequence repeat) was used to study the 5 populations. A total of 92 polymorphic loci were obtained from 10 primers which have been screened and P% is 67.4%. The genetic distances were among 0.0863 to 0.1454.To the two molecule marker systems applied in this study, the 5 populations and one Sargassum horneri as out-group were analyzed. Here the genetic distances of between the S. horneri and the 5 populations were remarkable compared with the genetic distances among the 5 populations. Furthermore, the genetic distances of two populations which were both"Lufeng No.1"collected from different years were consistently the minimum value in both RAPD and ISSR. Our results provide the tools for the Hizikia's genetic breeding and germplasm identification in the future... |
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