| Quality improvement of photo-thermo-sensitive male sterile line is an important foundation of quality breeding of two-line system hybrid wheat. It is significant to detect new gene/QTL of photo-thermo-sensitive male sterile line by using the technology of molecular marker for wheat quality breeding program of photo-thermo-sensitive male sterile line.In the present study, a total 289 doubled-haploid lines derived from the cross of photo-thermo sensitive genical male sterile line BS20 and Fu3 was planted in Beijing and Anhui respectively. Kernel protein contents, SDS sedimentation value, Grain hardness, Mixograph and RVA parameters were evaluated. A genetic linkage map was constructed with 158 SSRs markers.QTL analysis was conducted with the software QTL Cartographer2.5. Major results are described below:Four QTLs were detected for kernel protein content on chromosomes 1A,3A and 5D,in which the QTL on chromosome 5D was found both in Beijing and Anhui,but was mapped on the different region ,explaining 12.32% and 8.53% of the phenotypic variance,and the QTLs on chromosome 1A and 3A were detected only in Beijign account for 7.18% and 4.17% of phenotypic variance, the QTL on chromosome 1A and the marker Glu-A1 of genetic distances of 0.1cM .Six QTLs were found for SDS sedimentation value on chromosomes 1A,1B,3B and 5D,and the QTLs on chromosome 5D was detected across two environments,explaining 9.80% and 11.22% of phenotypic variance.In addition, two different QTLs for SDS sedimentation on chromosome 1A and 1B were found both in Beijing and Anhui respectively, accounting for 7.55%,7.61% and 18.30%,5.80% of phenotypic variance, and the QTL on chromosome 3B was detected only in Beijing accounting for 8.31% of phenotypic variance.Two QTLs were found for grain hardness on chromosome 1A and 5D, and the QTL on chromosome 5D was detected across two environments accounting for 75.68% and 75.30% of phenotypic variance,the QTL on chromosome 1A was detected only in Beijing explaining 3.73% of phenotypic variance.Six QTLs for mixing time were mapped on chromosomes 1A, 1B, 2B, 6B and 6D, respectively,explaining 5.13%-10.41% of phenotypic variance, in which one QTL on chromosome 2B was found in two environments explaining 9.35% and 8.06% of the phenotypic variance. For peak value,four QTLs were detected on chromosomes 1B,5D and 7B, and the QTL on chromosome 5D was mapped in two environments accounting for 7.88% and 15.48% of phenotypic variance. Two diffident QTLs were detected on chromosome 1B for peak value, accounting for 14.77% and 6.50% of phenotypic variance, and the QTL on chromosome 7B was detected only in Anhui accounting for 4.62% of phenotypic variance. Eight QTLs for peak integral were mapped on chromosomes 1A, 1B, 2B, 3B,5A, 5B and 6D, explaining 4.42%-15.19% of phenotypic variance,and the QTL on chromosome 2B was detected in two environments accounting for 7.52% and 7.33% of phenotypic variance. Two diffident QTLs were detected on chromosome 1A for peak integral, accounting for 4.83% and 5.40% of phenotypic variance. Six QTLs were detected for eight minute width on chromosomes 1A,1B, 2A and 3D, in which two differdent QTLs were found on chromosome 1B and 2A respectively both in Beijing and Anhui explaining 14.89% and 41.0%, 7.31% and 3.21% of phenotypic variance. In addition,one QTL on chromosome 1A was found in Beijing and one on chromosome 3D was found in Anhui accounting for 5.62% and 4.70% of phenotypic variance.For peak width, three QTLs were detected on chromosome 1B and 7D, and two differdent QTLs were found on chromosome 1B in two environments explaining 16.30% and 7.56%. In addition, one QTL on chromosome 7D was found only in Beijing accounting for 7.44% of phenotypic variance.Two QTLs were found , respectively, for peak viscosity, final viscosity and peak time on chromosomes 1A and 2B, 2B and 4A, 2A and 5B, explaining 6.48% and 7.02%, 5.98% and 4.70%, 6.04% and 5.31% of phenotypic variance.Only one QTL was found for breakdown on chromosomes 1A in Anhui, accounting for 5.39% of phenotypic variance. For setback,five QTLs were detected on chromosomes 1A , 2A, 2B, 4A and 7B,accounting for 5.53%-16.24% of phenotypic variance. No QTL was mapped on the same region in two environments. For trough viscosity, no QTL was found in two environments respectively.The QTLs on 1B near the marker Xcfd21 were associated with SDS sedimentation value, eight minute width,and peak width, indicating the big influences of this locus on these traits. In addition, the marker interval Xksum176.1~Xwmc59 on chromosome 5D influenced SDS sedimentation value, grain hardness, peak value significantly. The QTLs for mixing time and peak integral on chromosome 2B were also mapped on the same location. The QTLs were detected in differdet environments,with stable effeets on the traits.
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