Effects Of Alfalfa Saponins On Performance,Cholesterol And Cecal Microbe Of Laying Hens

The trial is designed to observe the effect of diet supplemented different levels of Alfalfa Saponins on Performance,Cholesterol and cecal microbe of Laying Hens. To make clear whether high-level Alfalfa saponins could influence egg cholesterol,and the preliminary discussion to their mechanism was indicated.These data provide practical basic for the Alfalfa Saponins in laying hens production.Hy-line brown laying hens(400days 288)were randomly divided into 4 groups,with 4 replications per group and 18 hens per replication.The control group fed with basal diets, the testing group fed with basic diets adding 30mg/kg, 60mg/kg, 90mg/kg Alfalfa Saponins respectively. Pretest period was10 days and experimental period was60 days. The main results were showed as follows:①The diets added with Alfalfa Saponins did not significantly affect feed intake,the rate of laying,egg weight,rate of feed and egg(P >0.05).②The diets added with Alfalfa Saponins could improve semi-rate.Ⅲgroup was best(P<0.05); ventral fat rate ofⅢ,Ⅳgroup was reduced significantly(P<0.05). the slaughter rate,the whole-rate,Spleen/the whole weight were not improved significantly (P >0.05),③The diets added with Alfalfa Saponins could significantly improve the Hulf unit.Ⅲg roup was best(P<0.05); eggshell thickness was increased significantly(P<0.05). The diets added with Alfalfa Saponins did not significantly affect the egg-shaped index and yolk color(P>0.05).④The diets added with Alfalfa Saponins 30 days could significantly reduce the contents of cholesterol in egg yolk ofⅢ,Ⅳgroup(P<0.01); did not significantly affect the contents of cholesterol in egg(P>0.05).The diets added with Alfalfa Saponins 60days could significantly reduce the contents of cholesterol in egg yolk ofⅡgroup(P<0.05), could significantly reduce the contents of cholesterol in egg yolk ofⅢ,Ⅳgroup(P<0.01); could significantly reduce the contents of cholesterol in egg ofⅢ,Ⅳgroup(P<0.01), could significantly reduce the contents of cholesterol in egg yolk ofⅢgroup toⅡgroup(P<0.05). The diets added with Alfalfa Saponins did not significantly affect yolk weight,egg weight (P>0.05). The diets added with Alfalfa Saponins could significantly reduce the contents of cholesterol in liver ofⅢgroup(P<0.01), could significantly reduce the contents of cholesterol in liver ofⅣgroup(P<0.05), could significantly increase the contents of cholesterol in dejecta ofⅢgroup(P<0.05).⑤The diets added with Alfalfa Saponins could significantly reduce the contents of cholesterol in the blood.Ⅱ,Ⅲ,Ⅳgroup could significantly reduce the contents of cholesterol in serum ofⅡ,Ⅲ,Ⅳgroup(P<0.01); could significantly reduce triglyceride,low density in serum ofⅢ,Ⅳgroup(P<0.05); could significantly reduce phosphorus in serum ofⅢ,Ⅳgroup(P<0.05); The diets added with Alfalfa Saponins did not significantly affect high density lipoprotein,total Protein,albumin(P>0.05); did not significantly affect GOT,GPT,AKP,LDH and BUN(P>0.05).⑥The diets added with Alfalfa Saponins could significantly reduce the contents of colibacillus in caecal ofⅡ,Ⅲ,Ⅳgroup(P<0.01); could significantly increase the contents of bacillus bifidus in caecal ofⅢg roup(P<0.05).⑦The diets added with Alfalfa Saponins could significantly reduce the contents of N in dejecta ofⅡgroup by 12%(P<0.05),Ⅲgroup 21.8%(P<0.01),Ⅳgroup 15.3%(P<0.01).The diets added with Alfalfa Saponins could significantly reduce the contents of NH3 in dejecta ofⅢgroup(P<0.01), could significantly reduce the contents of NH3 in dejecta ofⅣgroup(P<0.05). The diets added with Alfalfa Saponins did not significantly affect the contents of P in dejecta(P>0.05).