Study On Gene Expression Of Uppermost Internode In Eui Mutant Of Rice (Oryza Sativa. L)

Changxuan 3S was obtained from thermo-sensitive genic male sterile line(TGMS) Pei' ai64S. It is sensitive to gibberellins and is controlled by single recessive gene. In this research, the gene expression patterns in elongated uppermost internode of TGMS eui mutant Changxuan 3S through oligonucleotide microarray and semi-quantitative PCR, using Pei' ai 64S as control. The data obtained in this research are mainly as follows:Firstly, we found 545 differentially expressed genes on the genechip with 60,000 hybridization sites representing 36,926 transcripts. Among these differentially expressed genes, 362 genes were up-regulated, and 183 genes down-regulated, which accounted for 66.42% and 33.58% separately of total differentially expressed genes. The result of further semi-quantitative RT-PCR analysis showed the reliability of the result of microarray analysis.Secondly, we found that the differentially expressed genes could be grouped into seven categories according to bioinformatics analysis. That was: signal transduction-associated genes, membrane and transport associated genes, cell growth and apoptosis associated genes, replication and repair associated genes, carbohydrate metabolism associated genes, biosynthesis of secondary metabolites associated genes and others with unknown function. In signal transduction-associated genes, carbohydrate metabolism associated genes and biosynthesis of secondary metabolites associated genes, we found lots of internode elongation related genes. eui gene may affect these gene expression to achieve rapid elongation of uppermost internode.Thirdly, we further analyzed two genes differentially expressed greater than 10-fold. It was showed that they were genes with unknown function, but there were hormone-responsive elements in their promoters. So, they might involve in hormone signal transduction. We designed specific primers according to their gene sequences, and cloned the cDNA sequences containing the whole open reading frame of the genes by RT-PCR. Their cDNA sequences are identical with 9311' s, but different from Nipponbare' s. Then we constructed gene over-expression vectors and delivered the genes into Arabidopsis successfully. The analysis of the transgenic Arabidopsis is in progress.