Research On Classify Identification Of Auricularia Auricula Strain By Morphology And Biochemistry Method

It is very important to solve the confusion state on Auricularia auricula strain by set up reliability simple identification system under(this) circumstances that there is no consummate domestic fungus registration system in our country.The classify identification was done to 23 strains of Auricularia auricular which were widely planted in north-east area.Through choosing indexes,we want to find Stabile and detailed condition to let this foundational method masting by more people,Compared to expensive and complicated molecular biological method.1,Esterase isozyme electrophoresis divided 23 strains into 13 types.the first type is number 4,5,16,23;the second is 12 and 14;the third is 13;the fourth is 20;the fifth is 6;the sixth is 7;the seventh is 11;the eighth is 10,18,17,19,21,22;the nineth is 3;the tenth is 8;the eleventh is 1;the twelfth is 15;the thirteenth is 9.Esterase isozyme electrophoresis is feasible classify identification, method.it should be generalization.2,Dissolubility protein electrophoresis divided 23 strains into 5 types.the first type is number1,2,3,12,14;the second is 4,5,6,11,16,23;the third is 7,8,19,21,22;the fourth is13,15,20;the fifth is 9,10,17,18.Although categorization is not very exquisite,but it can raise experiment accuration together with Esterase isozyme electrophoresis.3,Peroxydaset isozyme endocellular enzyme polyacrylamide gel electrophoresis is inconcinnity classify identification of Auricularia auricula strain.4,At the rivalry essay,There are 252 groups,and have 175 groups are consistent with the fuzzy grouping analysis result of the Esterase isozyme electrophoresis,for instance,at the fuzzy grouping analysis,belong to the diff-congeneric strains:13and 14,6and 7,1and 6,7and 14,16and 17,13and 18,13and 18,19and 14 have the visible rivalry line,coincidence rate achieved 70%. itdue to the solubility protein categorization essay is not very exactly,this text hasn't contrast to the result rivalry essay.at the essay of difference,1and 17 has the biggest difference of difference,,which achieved 6days,it is identical to the electrophoresis essay,the dissimilarity coefficient are biggest in the fuzzy grouping analysis,at the same time,the solubility protein level have large diference..that explained although the method of morphology and the solubility protein level metered have relative accuracy,they could aid and addition the essay,and have the promotion to the results.so they can use in the classify identification of the Auricularia auricula strain.