Effect Of The Transgenic Sugarcane On The Major Pests Of Sugarcane And Natural Enemies Of The Major Pests

Field investigation and laboratory experiments were carried out to test the effect of the transgenic sugarcane on the population dynamics of the major insect pests Mythimna separata and Ceratovacuna lanigera Zehntner in sugarcane fields,and biological responses of the major pest Mythimna separata,Ceratovacuna lanigera Zehntner and predatory natural enemy Ladybug sexmaculates to the transgenic sugarcane directly and indirectly,with conventional sugarcane as the control. The results were as follows:1 Effect of transgenic sugarcane on the major pest M. separata .Transgenic sugarcane had higher resistance to M. separata.The population density of M. separata and damage rate of plants or leaves in the transgenic sugarcane field were all significantly lower than those in the control field.Biological test in lab showed that at different growing stage of sugarcane, the transgenic sugarcane could significantly adversely affect the survival,development, pupation and fecundity of M. separata,while had no significant effect on the preference of feeding and oviposition,pre-spawning and life span of male and female moths.The activity of carboxlesterase (CarE)of M. separata was lower when feeding on transgenic sugarcane than that on CK,while no significant difference of acetylcholinesterase (AChE)and Glutathione S-transferase was found.2 Effect of transgenic sugarcane on major t pest C. lanigera Zehntner.Transgenic sugarcane had no significant effect on parameters of Ceratovacuna lanigera Zehntner, such as population dynamics,body weight,volume of honeydew, feeding preference,generation cycle,oviposition choice and activity of CarE,AchE and GSH.3 Effect of transgenic sugarcane on the predatory natural enemy L.sexmaculates.Effect of transgenic sugarcane on the bionomical characteristics of L.sexmaculates through its preys Ceratovacuna lanigera Zehntner were studied in the lab,with larvae of Ceratovacuna lanigera Zehntner fed on transgenic sugarcane for a given time as preys.The results showed that there was no difference in the mortality of different ages ( except the pupal stage ),the developmental period of different ages, adclt weight,emergence rate and sex ratio;Both predatory functional responses of the Ladybug followed the Holling II model.All the activities of AchE,GSH and CarE in Ladybug were obviously enhanced under transgenic sugarcane treatment,except for AchE in the third week.