The Evaluation Of ScMV-CP Gene Transgenic Sugarcane

Abstract Sugarcane mosaic disease is one of major disease to sugarcane which was cuased by the sugar cane mosaic virus and influences the growth of sugarcane seriously. Breeding resistant cultivars by genetic engineering is an assistant method in high-effective breeding of sugarcane. ScMV-CP gene was transformed to high-susceptible sugarcane cv. Badila by particle bombardment in the Key Lab of Sugarcane Eco-Physiology& Genetic Improvement, Ministry of Agriculture in 2003, and 13 ScMV-CP gene transgenic sugarcane were gained. After,5 transgenic lines were chosen for environmental release according to the agronomic performance and resistance of transgenic sugarcane.Using these transgenic sugarcanes as material, the resistant stability and agronomic performance of transgenic sugarcane were studied after environmental release; Through years of environmental release of multi-point test.The results showed as followed:Resistance to ScMV was improved significantly in CP transgenic sugarcanes.and B48 which is only in the incidence of 2.64%, the other four transgenic sugarcanes but also disease-resistant, mosaic disease incidence of less than 30%, while the incidence of the original species of close to 100%.Mosaic virus as a result of anti-power, The yield and quality traits of these transgenic sugarcanes are improved.Compared with the original species, transgenic sugarcanes ' production increased 13.7% ~ 93.6%, Sugar to increase the absolute value of 0.07% ~ 1.25%.Through multi-year stability of multi-point analysis,B48 and B51 all have good performance.Traits through the arts, industry and agriculture for the stability analysis,The transgenic line B48 was the best line in 13 transgenic sugarcane lines, and had nice agronomic traits and no disease development after inoculation or natural infection in three years.