The Study Of Concentration Of Bacillus Thuringiensis Fermentation Browth And Microencapsulation Of Pesticide Protein Of Bt

It studied air lift fermentation for Bt LSZ9408, the centrifugal filtration technology conditions of fermentation broth, the flocculation concentration technology condition of chitosan as flocculant for concentrating fermentation liquid. The optimization technology for preparation Bt pesticide protein to Enteric-coated microcapsules dosage form, the mensurating methods of protein envelopment quantum of hydroxy proprl methyl celluloxe phthalate protein microcapsules, un-enteric-coated microcapsules of Bt pesticide protein and the released character of enteric-coated microcapsules or not for Enteric-coated microcapsules were also studied in it.Many regular crystals of pesticide protein were obtained by air lift fermentation. The centrifugal filtration could eliminate big particles, which made for separation and purification of industry of Bt pesticide. The optimum technology condition was 1 L disposal quantity every time, temperature 10-15℃,pH 4.5. It could avoid the effect of chemical or microbial flocculant on active compound if chitosan as flocculant, which can remove about 75% water or coloured material, the optimal technology condition was pH5.0-6.0, temperature 30-35℃, chitosan concentration 0.025%, electromagnetic stirring time 5 min, electromagnetic stirring speed 550r/min. By separation fermentation liquid of Bt, which obtained that the pesticide protein purity extracted by isoelectric point precipitation method was higher than organic solvent method.Folin-pHenol assay was the appropriate method for detecting protein envelopment quantum of HPMCP microcapsules. The shape of enteric-coated microcapsules prepared by two pHase emulsion assay was rotundity and un-conglutinant, the optimum technology condition was dispersed pHase 0.5%, stirring speed 6000r/min, wall material 2%-3%, emulsifier concentration 5%, heartwood concentration 4%, dispersed proportion 1:2.5-1:3.5. The shape of un-enteric-coated microcapsules ethyl cellulose microcapsules was regular article and un-conglutinant. By the released character study of microcapsules which obtained that the released quantum reached peak value in two to three hours in vitro test, the enteric-coated released more quickly in alkalescence solution than in acidity solution but the un-enteric-coated was not.