Analyses Of The Differential Expressed Genes During Colonizing And Developing Of Magnaporthe Grisea In Rice Plants

The system of rice- Magnaporthe grisea, is an ideal model system of the plants interaction with their pathogens and the ecological control of plant disease. In order to understand the interaction molecular mechanism between Magnaporthe grisea and rice, the specific expression genes during the pathogen infection were screened out by the technology of gene chip and some of them were verified by RT-PCR.The result of gene chip experiment showed that, for more than 2-fold change, there were 3309 up-regulated genes and 3453 down-regulated genes from Magnaporthe grisea; and 974 up-regulated genes and 1134 down-regulated genes from rice. The NCBI, Magnaporthe grisea genome and Rice Genome Annotation Project databases, and ProtComp 8.0 software were used to annotate the proteins and their subcellular localizations of the most obviously changed genes, in which122 up-regulated and 66 down-regulated genes from M. grisea, and 62 up-regulated and 66 down-regulated genes from rice. Finally, the top 20 up-regulated genes were selected from Magnaporthe grisea and verified by RT-PCR analysis.