| Aureobasidin A (AbA),a cyclic nonapeptides secreted by Aureobasidium pullulans R106. It is a broad-spectrum antibiotic and is functional in many fungi and protozoa. It can also inhibit the growth of fungi through inhibiting the synthesis (transfer) enzyme of muscle glycogen phosphatidyl(IPC),while the gene locus-specific mutation of the enzyme will lead to the bacterial resistance to AbA.In this paper, the mutants for resistanting AbA are obtained through ultraviolet radiation and screening AbA. The synthase genes of mutant Botrytis cinerea IPC are amplified. The similarities and differences between the synthase genes of wild-type Botrytis cinerea IPC and the amino acid sequence and the sequence of mutant are compared. It is found that the the functional domain of the interaction between the synthase of Botrytis cinerea IPC and AbA lies in the C-terminal. The increase of alkaline and the hydrophobicity enhancement of the mutant IPC synthase are favorable for the combination with the hydrophobic weak acid AbA, which may be related to the generated resistance to AbA.AbA can effectively inhibit the dissemination of the Botrytis cinerea. The secreted quantity of mycophenolate acid that Botrytis cinerea and mutant with AbA, the hydrolysis activity and the impact of disease index of tomato are measured, which shows that AbA can reduce the activity of Botrytis cinerea, the themycophenolate acid associated with the disease and the hydrolytic enzyme, thus weaken the disseminated force to the plant.AbA could inhibit the growth of Botrytis cinerea. The experiment about the influence of AbA to the Botrytis cinerea spores, germ tube and the growth of mycelialthe indicates that AbA can inhibit the growth of Botrytis cinerea though affecting the polar germination of the g Botrytis cinerea spores, the elongation of germ tube and the morphological changes of the mycelium. |
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