Isolation And Characterization Of A Virulent Strain Of Canine Adenovirus Type 1 And Primary Study On Dog Experimental Infection

Canine adenovirus type 1(CAV-1) can cause infectious canine hepatitis and encephalitis of bear and fox. Its infectious rate in dog is over 50%, and the death rate reaches at 25%⁴0%. It seriously threaten to canine and fur economical animals. Vaccination is the primary way to prevent from infection. Unfortunately, there are only canine 5-mixed and imported vaccines at present. It is necessary to devolop a special vaccine for fur economical animals. Thus we need a standard virulent strain of CAV-1 as a judgement of vaccine.A virulent strain of canine adenovirus (CAV) was isolated and identified based on nomal means. It has a high homology in partial fragment of AE3 region by comparing with standard strains of R1261 and Glaxo, respectively at 99.7% and 98.9%. And we proved its pathogenicity through dog infcetion test. The infected dogs had typical clinical symptoms of CAV, and had certain regularity in physiological and hematologic index. At last, we make a conclusion that 2×10⁶ 50% tissue culture infective dose(TCID₅₀) units per kilogram weight was minimum lethal dose to these dogs.We provide a standard virulent virus for testing vaccine efficacy of dog including some economical animals after our work.