Genetic Diversity And Germplasm Resources Conservation Of Different Torreya Grandis Fort Ex Lindl

Torreya grandis Fort.ex Lindl is evergreen arbor species,which belong to Taxaceae and Torreya.It is China′s unique high-quality dry fruit,it has high economic value. Phenotypic identification and amplified fragment length polymorphism (AFLP) were used to analyze the genetic diversity of 92 Torreya grandis Fort ex Lindl from 6 different populations. Which can provide a scientific and rational theoretical basis for protective strategy of diversity, germplasm resources conservation and screening of superior germplams. The main results are as follows:1 Phenotypic diversityThe CV of six groups of phenotypical traits, which are the length, width, weight of fruit and seed, the length ,width of leaf are 14.15%, 3.95%, 33.92%, 15.44%, 15.77%, 34.71%, 18.85%, 15.48%.The average of Vst among populations is 10.8%, with the length, width, weight of fruit and seed, the length ,width of leaf are 11.9%, 19.1%, 0.1%, 16.7%, 14.6%, 10.8%, 12.0%, 1.0%. Analysis of variance showed that the variance component within populations (72.8%)is sligntly greater than that of among populations(8.7%).2 Genetic diversity of different populations by AFLPAmplified fragment length polymorphism (AFLP) was used to analyze the genetic diversity of 92 Torreya grandis Fort ex Lindl from 6 different populations. Total of 364 locis of Torreya grandis genome were examined for molecular variation and 63 locis were polymorphic. Analysis of variance showed that the percentage of variance components was 11.14% among populations and 88.86% within populations. The percentage of polymorphic loci(P)ranging from 79.73% to 98.41%,the Nei′gene diversity(H) ranging from 0.3096 to 0.3768.the number of alleles(A)ranging from 1.7939 to 1.9841,the effective number of alleles(Ae)ranging from 1.5416-1.6759,Shannon information index(I)ranging from 0.4548 to 0.5471.As for Torreya grandis Fort ex Lindl,genetic differentiation coefficient among 6 populations (Gst)is 0.1136. The gene flow among 6 populations(Nm)is 3.8997. The average genetic identity is 0.9174 and the average genetic distance is 0.0797. The Torreya grandis Fort ex Lindl has a high diversity and the genetic variation is mainly concentrated within populations.3 Comparison of the two results showed that , the correlation coefficient between phenotypic diversity and Genetic diversity of different populations by AFLP is 0.9783, which proved the consistency of the two results.