| Japanese encephalitis was one kind of central nervous system's acute infectious disease after mosquito bite dissemination threaten seriously human and animals health. The pig was Japanese encephalitis virus's important storage host and increases the host, and was it's main origin of infection. To prevent Japanese encephalitis's unpopularity effectively, protects the human and animals security, has conducted the investigation and study to the Henan Province pig infection encephalitis virus situation.This experiment maked the appraisal first from the serology aspect to Japanese encephalitis virus's immunity protection situation, also had carried on this virus's separation appraisal from the molecular biology aspect to the suspicious case. Since May, 2008, my laboratory had collected 801 blood serums from 12 city certain pig farms which already immunitied Japanese encephalitis of Henan Province, the JEV IgG immune body were examinatied by pig Japanese encephalitis virus ELISA immune body examination reagent box and carried on the analysis pig Japanese encephalitis popular situations of HeNan province. During 2008 year, the 22 doubtful Japanese encephalitis's material were seperated and charactrized from sickness morbidity pig farm's of The Henan Province various areas. With cell culture's method separation virus, carries on the RT-PCR appraisal to the new separation's virus. The result is as follows1.â‘ the Japanese encephalitis antiserum body masculine rate was 47.94% of our province, In some areas pig farms the immune body masculine rate was only 10.00%. Because after examines the blood serum is the vaccine immunity blood serum, therefore, entire province pig farm immunity protection ratio insufficient 50%, is generally low, which are in high-risk send the area.â‘¡Different seasons from the contrast, In 5,6 month the positive rate of Japanese encephalitis was low, less than 40%, and in August the highest serum antibody positive rate, reaching 55.99% , followed in July, reaching 47.50%, which are probably farms in general in the summer to take the results of immunization.â‘¢From the province of all ages JE Pig serum antibody test results found that sows the serum antibody positive rate reached 78.67 percent, boar serum antibody positive rate of 75.00%, but breast-feeding, conservation, fattening Piglet serum antibody positive rate low, From this we can see that the male and sow positive rate was high mainly because many times the result of immunization, which were concern to the immunity protection ratio and the vaccine quality, the pig body belt poisonous intercross infection and the technician operating. But Newborn piglets, nursing piglets and fattening pig's masculine gender rate to be low, the possible reason was that the newborn pig organism resistivity was bad, or abuses the medicine, caused the partial newborn pigs to infect JEV, created the immunity defeat or immunosuppression. Therefore, to guarantee that humanity's security, the pig-breeding industry healthy development, besides strengthens to the grown-up pig's immunity, must to newborn pig's prevention protection, prevent the occurrence which with emphasis the pig JE got sick and popular.2. From the collection of 22 sickness materials and successfully isolated a virus, named HeN1. The PK-15 cell had the cell pathological change which caused by the HeN1 virus,the time of the CPE was 2~4d. In order to Analyzes this poison for the further, one pair primer was to be desiged, Japanese enceph alitis virus were to be examined by the RT-PCR. the goal was carried out. carried on the homology analysis with the DNAstar software to these 13 JEV genes, the sequence was the target sequence. The result indicated: new separation's JEV HeN1 with downloads from GenBank what the JEV poisonous sequence homology was 96.6%, the cladogram analysis indicated that separated poisonous newly with other poison not in the identical branch, this possible to concern with this viral existence certain variation.
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