The Establishment Of Regeneration System On Begonia Semperflorens And Begonia X Hiemalis Fostsch

The adexual propagation system of Begonia Semperflorens and Begonia x hiemalis Fostsch were established in this research,with material of leaf blade and way of organogenesis,moreover,the most suitable tissue culture condition of two kinds of Begonia plants was compared.1.Disinfection Time:The most appropriate disinfect time processed by HgCl₂ of 0.1% was studyed,The results show that disinfect for 5min suit Begonia Semperflorens,disinfect for 6min suit Begonia x hiemalis Fostsch.2.The best Inoculation Period:This research has discussed the best inoculation period of two kinds of Begonia plant,inoculation period is January,March,May,June,October,November and December,it shows that the best inoculation period of Begonia Semperflorens is November to the first ten days of December;and Begonia x hiemalis Fostsch is October.3.Primary Culture:This test study the optimum primary culture medium with three factors and three levels of the orthogonal experimental design,the optimum initiate cultivate medium for Begonia Semperflorens is 1/2MS+6-BA0.5mg/L+IBA0.8mg/L;for Begonia x hiemalis Fostseh is 1/2MS+6-BA1.0mg/L+NAA0.4mg/L.4.Successive transfer Culture:In this paper,6-BA match with NAA and IBA respectively to research the influence of hormone to proliferation of two kinds of Begonia plants.The best proliferation cultivate medium of Begonia Semperflorens is MS+6-BA0.5mg/L+NAA0.3mg/L;of Begonia x hiemalis Fostsch is MS+6-BA1.0mg/L+IBA0.5mg/L.5.Root Generating and Seedling Training Culture:Based on the result of liquid rootage culture,solid root culture was researched in detail.The result indicate that the best rootage culture medium for Begonia Semperflorens is liquid culture medium with MS+GA₃0.5mg/L+NAA0.5mg/L+sucrose30g/L;for Begonia x hiemalis Fostsch is solid culture medium with MS+IBA1.0mg/L+sucrose 20g/L. 6.Transplant and Seedling:In order to choose the best transplant method,diffenent kinds of root was watering with the second day-planting,flooding method and traditional watering method in this test,the comparison shows that the plantlet of Begonia Semperflorens obtained from liquid culture plant the second day survive most;the plantlet of Begonia x hiernalis Fostsch obtained from solid culture that withour AC suit the traditional watering method.