Studies On Leafstalks (Leaves) In Vitro Culture And Plant Regeneration Of Plum

'Nubiana' plum (Prunus salicina. 'Nubiana' ) and myrobalan (Prunus cerasifera) were used as the materials in the study. By testing the optimal plant growth regulators , their combinations , basal medium combinations and culture conditions, the regeneration system was established using the leafstalks as explants. We also studied plant growth regulators and their combination, basal medium which affected on the callus inducing myrobalan leaves in vitro .The main results were as follows:Firstly, the optimal medium for leafstalks culture of 'Nubiana' plum was WPM+2,4-D 0.2 mg/L+TDZ 1.65 mg/L+AgNO₃ 0.5 mg/L, by 31.7% of adventitious bud rate. The best duration of dark was 14 d.Secondly, the optimal medium for leafstalks culture of myrobalan was MS+2,4-D 0.2 mg/L+TDZ 1.80 mg/L+AgNO₃ 0.5 mg/L, by 36.7% of adventitious bud rate. The best duration of dark was 21 d.Thirdly, through analysis of the effect of Ad concentrations on explants multiplication and growth, the optimal concentration of Ad for 'Nubiana' plum and myrobalan was 1.0 mg/L.Fourthly, the medium of 'Nubiana' plum shoot rooting was 1/2 MS + IBA 0.5 mg/L. The medium of myrobalan shoot rooting was 1/2 MS + IBA 1.0 mg/L.At last, the optimal medium on callus induction of myrobalan leaves was MS+6-BA 1.0 mg/L+2,4-D 2.0 mg/L, the inductivity reached 93.3%.