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Detection Of Yield And Salt-tolerant QTLs By Using Selected Backcross Introgression Lines In Rice (Oryza Sativa. L)

Posted on:2010-02-28Degree:MasterType:Thesis
Country:ChinaCandidate:Y L QianFull Text:PDF
GTID:2143360302957958Subject:Crop Genetics and Breeding
Abstract/Summary:PDF Full Text Request
It's quite difficult to manipulate the yield traits of rice due to the low inheritance,interaction with environment and the complex biology elements.Using the advance selective backcross introgression lines to take apart complex quantity traits and improvement of goal character is abroad applied in crop molecule breeding in our country.The experiment selects basic varieties whice is popularized widely as current parents to construct advanced backcross lines and go along with yield selection.To proceed the QTL and main points effect analysis is about the spikelet fertility, thousand grains weitht, panicle length, the number of filled grains per panicle, total number of spikelets per panicle, the number of panicles per acre, grain yield per plant and plant height of rice yield traits.At the same time,to proceed the salt-tolerance traits identify and QTL orientation of score of salt toxicity and survival days of seedlings with the selected introgression lines it to set forth how to apply the output of the QTL orientation in the manufacture practice and to cultivate high-yield with salt-tolerance new varieties.It's to applied the foreground of different background,the effect of the overlap of the yield with salt-tolerance traits and the integration foreground using of the every point of overlap.1. To proceed the QTL and main points effect analysis is about the spikelet fertility, thousand grains weitht, panicle length, the number of filled grains per panicle, total number of spikelets per panicle, the number of panicles per acre, grain yield per plant and plant height of rice yield traits.Forty nine QTLs of eight goal traits are detected in the line of Shuhui527/ZDZ057.The eight traits QTLs are nine,twelve,four,five,five,two,two and ten separately.It's distributed in the eleven chromosomes except for the eighth chromosome. Sixty one QTLs of eight goal traits are detected in the line of Minghui86/ZDZ057.The eight traits QTLs are fifteen,eleven,eight,nine,three,two,five and eight separately.It's distributed in the eleven chromosomes except for the eighth chromosome. Fifty four QTLs of eight goal traits are detected in the line of Shuhui527/Teqing.The eight traits QTLs are nine,ten,eleven,five,seven,one and eleven separately.It's distributed in the twelve chromosomes. Thirty one QTLs of eight goal traits are detected in the line of Minghui86/Teqing.The eight traits QTLs are two,nine,five,four,four,five and two separately.It's distributed in the nine chromosomes except for the eighth,ninth and twelfth chromosomes.2. To analyse the interaction with QTLs and main points effect are about the spikelet fertility, thousand grains weitht, panicle length, the number of filled grains per panicle, total number of spikelets per panicle, the number of panicles per acre, grain yield per plant and plant height of rice yield traits.In the level of P<0.0001,there are 122,161,2,5,1,1,1 and 4 pairs interact points in the line of Shuhui527/ZDZ057. And there are 58,123,4,68,53,12,2 and 15 pairs interact points in the line of Minghui86/ZDZ057. There are 263,59,72,12,6,0,0 and 93 pairs interact points in the line of Shuhui527/Teqing.And there are 1,9,4,6,20,0,0 and 3 pairs interact points in the line of Minghui86/Teqing.3. The first,third and seventh chromosomes are worth attendance to research genetic base of rice yield traits.There are 30 QTLs in the first chromosome,45 QTLs in the third chromosome and 18 QTLs detected in the seventh chromosome of the four lines of Shuhui527/ZDZ057, Minghui86/ZDZ057, Shuhui527/Teqing and Minghui86/Teqing.4. The QSf3c(131.5 cM) in the third chromosome detected in the line of Minghui86/ZDZ057 is a main QTL impacting the spikelet fertility, thousand grain weitht and the number of filled grains per panicle.The contributions are 15.91%,15.64% and 15.18%.The point presents great add effect to the spikelet fertility, thousand grain weitht and the number of filled grains per panicle.The QSf3c presents great positive dominance in the trait of number of filled grains per panicle and controls other traits via the interact with few random points.5. The Contribution to the plant height of QPh7b(54.2cM) in the seventh chromosome detected in the line of Shuhui527/Teqing abtains 34.04%.Besides the same district of QPh7b and Ghd7 in the line of Shuhui527/Teqing and the same district of QSf3c and GS3 in the line of Minghui86/ZDZ057,the four QTLs which are QPh7a of plant height detected in the line of Shuhui527/ZDZ057, QTgwt3f of thousand grain weitht with plant height in the line of Shuhui527/Teqing, QTnsp1c of total number of spikelets per panicle with the number of panicles per acre in the line of Minghui86/Teqing and QPL3d of panicle length in the line of Minghui86/Teqing are the keystone of the future more settings detecting and near- isogenic lines research.6. A total of 11,15,11 and 6 QTL were detected in Shuhui 527/ZDZ 057, Minghui 86/ZDZ 057, Shuhui 527/Teqing and Minghui 86/Teqing respectively. Three pleiotropic QTL were found in the population of Minghui 86/ZDZ 057. There were 2 QTLs could be detected simultaneously in Shuhui 527/ZDZ057 and Minghui 86/ZDZ 057, 1 QTL in Shuhui 527/ZDZ057 and Shuhui 527/Teqing, and 1 QTL in Minghui 86/ZDZ057 and Minghui 86/Teqing. QSst2c,QSst10b,QSst12,QSds1b,QSst4b,QSst2d,QSst2e,QSds10,QSst1b,QSst10c,QSst10d and QSds4b explained 20.36%,24.06%,22.50%,28.45%, 21.39%,23.31%,34.92%,42.18%,20.29%,20.50%,21.31% and 26.27% of phenotypic variation respectively. The QSst12 showed negative overdominant effect, QSst4b and QSds10 showed positive overdominant effects, and QSst10b and QSst2d showed negative and positive partial dominant effects respectively.7. To analyse the interaction with QTLs and main points effect are about the score of salt toxicity and survival days of seedlings.In the level of P<0.005,there are 40 and 5 pairs interact points in the line of Shuhui527/ZDZ057. And there are 31 and 19 pairs interact points in the line of Minghui86/ZDZ057. There are 40 and 5 pairs interact points in the line of Shuhui527/Teqing.And there are 31 and 19 pairs interact points in the line of Minghui86/Teqing.8. The add effect of the nine main genes in the general breeding which are QSst2d,QSst10b,QSst12,QSds1b,QSst9a,QSst4b,QSst2e,QSst2f and QSds10 should be fully used . The alleles of QSst2d,QSst10b and QSst12 which are from the donor of ZDZ057 and the alleles of QSds1b and QSds10 which are from the donor of Teqing can be used to reduce the score of salt toxicity so as to heighten variety salt-tolerance. The alleles of QSds1b and QSds10 which are from the Minghui86 can be used to prolong the survival days of seedlings so as to heighten variety salt-tolerance.and the alleles of QSst9a and QSst4b which are from the Shuhui527 can be used to reduce the score of salt toxicity so as to heighten variety salt-tolerance.9. There are 12 overlaps detected from the background of ZDZ057 and Minghui86.The least overlaps are 3 locus and the most overlaps are 6 locus in the background of Shuhui527 and Minghui86 for ZDZ057. There are 5 overlaps detected from the background of Shuhui527 and Minghui86 for Teqing. The least overlaps are 3 locus and the most overlaps are 7 locus in the background of Shuhui527 and Minghui86 for Teqing.The exceptional frequency overlaps district of 17 locus detected in the four lines are the future research direction of near- isogenic lines.The contributions of the overlaps are greatly high in the corresponding lines.
Keywords/Search Tags:Rice, Selective backcross introgression lines, Yield traits, Salt-tolerance, QTL detection, Genetic overlap
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