Studies Of Effects On Degradation Of Chlorpyrifos-Degrading Bacteria O6 In Different Mutagenesis Methods

In this paper, the laboratory screening of a chlorpyrifos-degrading bacteria for the test materials to UV and ion implantation methods for mutagenesis, to deal with the strains screened had been HY1, HY2, HY3 and other mutant strains. Studied the effects of mutant growth factor; studied mutant kinetics; studied mutant enzyme and strain differences in electropHoretic bands.The main study results were summarized as follows:1. Through UV mutagenesis to O6, in biomass of 9×10¹¹CFU/ml, chlorpyrifos concentration of 10 mg·L^-1 cases,the two mutant strains increased the degradation rate of 84.88%,96.16% respectively.2. Mutagenesis through the ion implantation to O6 has been a mutant HY3, In biomass was 9×10¹¹CFU/ml, chlorpyrifos concentration of 10 mg·L^-1 cases, the mutant strain increased the degradation rate of 79.78%.3. At a temperature of 30℃, culture time for 48h circumstances, HY1, HY2, HY3 are the size of the growth pH7.0> pH8.0> pH9.0> pH6.0> pH5.0;4. In culture time for 48h, pH7.0 circumstances, HY1, HY2, HY3 are the size of the growth of 30℃> 35℃> 25℃> 40℃> 20℃;5. At a temperature of 30℃, pH7.0 circumstances, the growth of mutant strains reach the maximum in about 24h, and then entered the stationary pHase.6. In this paper, at different biomass and different time of degradation circumstances, degradation kinetics are in line with the level equation. In different degradation time on degradation kinetics of the study, the rate constants HY1, HY2, HY3 of are 0.1310 d^-1,0.1400 d^-1,0.1642 d^-1, half-life of 5.15d, 4.72d, 4.31d.7. The adoption of the original strain and the mutant enzyme electropHoresis analysis bands and found that extracellular enzyme electropHoresis bands did not change significantly. It can be that degrading enzyme exists in the cells...