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Study On Mechanism Of Magnesium Absorption And Photosynthesis Promotion In Citrus Incoulated AM Fungi

Posted on:2011-01-23Degree:MasterType:Thesis
Country:ChinaCandidate:W LiFull Text:PDF
GTID:2143360302997271Subject:Pomology
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The citrus is a member of Citrus Linn of Rutaceae. citrus is fruit cultivation in China Southern,production of citrus in the south which is the highest of all fruits, citrus cultivation in the south to help farmers increase the economic efficiency, thereby promoting the overall development of China's agricultural economy, on increasing farmers income, promote social stability important role in the development. Magnesium is an important element involved in chlorophyll synthesis, citrus magnesium deficiency show leaf chlorosis, necrosis and other symptoms, high light intensity in the outside world, the loss of green leaves, necrosis of more obvious symptoms. Citrus production, magnesium deficiency causes deficiency in addition to the soil itself, but also with the soil pH are too high magnesium ions into an insoluble form of the complex to citrus magnesium deficiency.Chongqing is one of the main producing areas of citrus, while the climate in Chongqing's own characteristics and weather information in Chongqing in August when five to weather conditions as high temperature, low rainfall in dry condition of soil, sunlight is sufficient. In September to December the weather information is displayed in Chongqing sunshine strong enough for the obvious "widowed" sunshine place. Chongqing has a unique light and weather conditions [5], these factors is an important condition for restricting the growth of citrus, citrus is easy to form mycorrhizal plants, easy to be all kinds of infection by arbuscular mycorrhizal fungi, and the citrus root on the other mineral elements and water absorption are largely dependent on mycorrhizal fungi. Arbuscular mycorrhizal fungi and host each other there is a certain selectivity, different fungi on different hosts with different infection rates, and different fungal infection of its citrus effect and growth promoting effects are also different. Therefore, in order to choose the right citrus arbuscular mycorrhizal fungi on citrus root for the absorption of mineral elements, play an important role to promote the growth of citrus. This test is for in Chongqing this "unique" weather conditions, by inoculating the surface Glomus Glomus versiforme (Gv), Moses Glomus Glomus mosseae (Gm) and light transmission Glomus Glomus diaphanum (Gd) to filter citrus the dominant species, and to study the antagonistic conditions different mycorrhizal fungi on citrus magnesium absorption, utilization and promotion of citrus photosynthesis effects, the set of three tests:Experiment 1 The filtration of preponderant Arbuscular Mycorrhizal Fungi of citrusTest 1 Test with potted rootstock Carrizo citrange annual (Carrizo citrange, Citrus sinensis Osbeck x Poncirus trifoliata (L.) Raf.) As test materials were inoculated versiforme Gv, Glomus Moses and transparency balloon Gm mold Gd, each dealing with three to a cell, with 3 repeats, randomized block arrangement. Study three different mycorrhizal fungi on citrus growth and development of infection conditions and the impact indicators to screen out the dominant species of citrus.The results showed that vaccination from the mycorrhizal fungi on citrus growth and development are stimulated after vaccination citrus height, ground dry weight, shoot dry weight were significantly higher than non-vaccinated control. And between the different fungi to promote the degree of difference, Moses Glomus inoculation the growth promoting effects of citrus most significant citrus height to 52.14±2.77cm, underground dry weight were 5.575±0.231cm, shoot dry weight 14.92±1.27cm. Test showed that Glomus Moses studied the dominant species of citrus species.Experiment 2 Study on mycorrhizal fungi on citrus photosynthesis and growth effectsObtained under the same management level annual rootstock Carrizo citrange (Carrizo citrange, Citrus sinensis Osbeck x Poncirus trifoliata (L.) Raf.), On citrus seedlings in soilless cultivation treatment of mycorrhizal fungi in the case of magnesium deficiency on Citrus absorb the impact of magnesium. Test consists of three treatments were imposed on citrus seedlings in nutrient solution without magnesium element, imposed on citrus seedlings with 40% of the amount of magnesium in the nutrient solution, applied to citrus seedlings with 100% total amount of magnesium nutrition. To non-inoculated as controls. Each treatment three to a cell, with 3 repeats, randomized block arrangement. The results show that citrus by citrus roots inoculated with bacteria can contribute to soil in the absorption of magnesium and magnesium deficiency in the appropriate context within the scope of VA mycorrhiza on citrus roots on the absorption of magnesium the most significant effect. Citrus seedlings with 40% of the amount of applied nutrient solution magnesium, citrus body magnesium content 112.37±4.87 mg·g-1.Experiment 3 Study on photosynthesis by increasing of AMF the effect on growth of citrus Obtained under the same management level annual rootstock Carrizo citrange (Carrizo citrange, Citrus sinensis Osbeck×Poncirus trifoliata (L.) Raf.), Total of three treatment experiment, the soil in the pH5.5-6.4 to grow immunized dominant strain of citrus seedlings, the soil in the pH6.5-7.4 to grow the dominant strain of citrus inoculated seedlings, the soil in the pH7.5-8.5 to grow the dominant strain of citrus inoculated seedlings, with no vaccination for the control. Each treatment three to a cell, with 3 repeats, randomized block arrangement.The results show that the same treatment in the arbuscular mycorrhizal fungi inoculation could promote the conduct of citrus photosynthesis, especially in the pH5.5-6.4 in the photosynthetic efficiency of the best soil, its photosynthetic rate averaged 5.234±0.561 (CO2m-2s-1) Protein content was 3.987±0.543 mg·g-1, soluble sugar content was 1.617±0.107 mg·g-1.
Keywords/Search Tags:Citrus, Arbuscular Mycorrhizla Fungi, Magnesium, Photosynthesis
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