Detection Of Pathogenicity Genes Of Magnaporthe Grisea And Distribution In Heilongjiang Province

The experiment utilized Molecular markers to identify and analysis Pathogenicity genes of Magnaporthe grisea in Heilongjiang Province,which were MPG1,MPS1,MagB,CPKA,MNH1,MgYCA1,MgS11and MgATG5.It clarified the distribution of the eight Pathogenicity genes and pathogenic ability of Magnaporthe grisea group in Heilongjiang Province.The results showed that the frequency of different Pathogenicity genes was significant different in Heilongjiang Province.The MPS1 had the most widely distributed with the positive frequency up to 52.94%; the second biggest distribution was the Pathogenicity gene MPG1 of which positive frequency was 37.13%; The positive frequencies for CPKA,MagB,MgATG5,MgYCA1 andMgS 11 were 33.74%,31.33%,29.48%, 25.75%and23.51%, respectively;The MNH1 was not detected here.Different the strains tested carried a significant difference in the number of Pathogenicity genes. Application Wolfe and Schwarzbach(1975) proposed methods will carry the same strain of pathogenic genes classified as a class, based on seven Pathogenicity genes in theory can be divided into 127 types of Pathogenicity genetic.The test detected a total of 64 pathogenic genotype type, five Pathogenicity genes carre 3 genotype type,The frequency was2.4%; four Pathogenicity genes carre 12 genotype type, The frequency was 10.8%; three Pathogenicity genes carre 21 genotype type, The frequency was 18.6%; two Pathogenicity genes carre 20 genotype type,The frequency was35.3%; one Pathogenicity genes carre 7 genotype type, The frequency was 25.7%; zero Pathogenicity genes carre 1 genotype type, The frequency was 7.2%; showing the structure of Magnaporthe grisea is more complex in Heilongjiang Province,but the genes that less Pathogenicity genes carrying mainly, most of the genetic basis of pathogenicity of individual is relatively simple and pathogenicity is not strong, so planting resistant varieties use control.The pathogenic genotype significantly different frequencies in Heilongjiang Province. The pathogenic genotype of higher frequency is 127,64,58,59,96,119,91and123, the second is 61,62,81,93,122 and 126. These pathogenic genotype widely distributed, but differed greatly among regions in Heilongjiang Province. In these type of genetic pathogenic,most of the genes carried by the number of relatively small,Its relatively simple genetic basis of genetic pathogenic.While carrying three Pathogenicity genes more than genotype is the lower frequencies in Heilongjiang Province, are in the local region, there is great difference between regions. Description structure of Magnaporthe grisea is more complex in Heilongjiang Province.The actual pathogenic genotype frequency and theoretical frequency difference larger, this may be related to the interaction between the Pathogenicity genes.The rice blast fungus range Magnaporthe grisea structure are quite different in Heilongjiang Province, the rice area has been formed with the characteristics of this region the structure of Magnaporthe grisea. This may be related to Rice Varietiesin Planting this region related to the structure.