Regulation Effects Of Plant Growth Regulators On Seedlings Building, Water Stress Of Alfalfa

Test in 2008 in Heilongjiang province Lindian County auspicious test base village and agronomic laboratories. In alfalfa (Medicago sativa L.) (Longmu 801, Prairie No. 1) as the test material, SODM, DTA-6, CC, DPC four plant growth regulators gradient solution as reagent, on alfalfa seed after soaking 8h , water soaking as CK, outdoor yard box by planting sand culture, indoor potted plants, vertical panels and roof are three experimental methods to study the PGRs on the completion of alfalfa seedlings and regulatory effects of water stress, reached the following conclusions:Outdoor planting in sand box method showed: the gradient of the solution of four regulators to take after sowing by seed, seedling alfalfa produced different promotion and inhibition effects of the four regulators, only DPC at high concentrations 400mg / L, 200mg / L Alfalfa seedling emergence under significant inhibitory effect (P <0.01), negative and promote seedling; integrated morphological and physiological indicators, regulators SODM: 15mg / L, DTA-6: 30mg / L, choline chloride (CC): 25 mg / L, DPC (DPC): 25 mg / L has the best seedlings for effect, showing the best of the seedlings into effect.In the four plant growth regulators soaking conditions, the alfalfa plant height, root length, surface material of the accumulation of ground substance accumulation and concentration effect shown, in general showed high concentrations inhibited also found that the DPC, CC in the concentration 400mg / L, DTA-6 at a concentration of 120mg / L, the severe phytotoxicity on alfalfa seedlings.The results show that the vertical plate: comparison with the control, regulator of choline chloride (CC) concentration of 200mg / L, the main root of the alfalfa seedlings into coarse, plant height, root length has the best inhibitory effect; while concentrations of 25 mg / L, the level of regulator substances on the accumulation of alfalfa seedlings significantly inhibited. Regulators SODM concentration of 15mg / L when alfalfa seedlings into the main root diameter, plant height, root length significantly promoted; concentration of 15mg / L when the material accumulation of alfalfa seedlings established a significant role in promoting. Regulator (DTA-6) concentration of 30mg / L, the main root of the alfalfa seedlings into coarse, plant height, root length and the accumulation of a significant role in promoting; regulator of DPC (DPC) concentration of 25mg / L when completion of the main root of the alfalfa seedlings rough, plant height, root length has the best control effect.In plants under drought conditions, results showed that: in most metrics, the grassland than 1 Longmu 801, outstanding performance, reflecting its excellent anti-stress quality. The plant growth regulator treatments, choline chloride treatment of alfalfa by continuing under water deficit in plant height, root length, dry matter accumulation increased significantly, holding high chlorophyll, free proline accumulation than the maintenance of high, showing an increase of choline chloride has a good effect of alfalfa resistance; other three kinds of plant growth regulators also showed a good effect, DPC was significantly increased for two species of plant height and stem diameter growth, and promote long grazing 801 root activity increased; DTA-6 significantly promoted the two cultivars, proline content and root length increased grassland 1; SODM significant for the two cultivars, proline content increased, stem diameter increased root length growth of grassland on the 1st, Long Mu 801 root activity increased.Flooding conditions in the pot, the results show that: on alfalfa, the grasslands on the 1st measurement indicators in the most outstanding performance reflects the performance of its excellent resistance to flooding. The plant growth regulators in plants treated by SODM sustained stress conditions in the flooded stem diameter, plant height, fresh weight to perform well, holding high chlorophyll, free proline accumulation to maintain a longer time accumulation to maintain higher than normal water conditions in the outstanding performance, the performance of the SODM good resistance to increase the effect of alfalfa. DPC significantly promote the two alfalfa varieties and stem diameter growth of proline, DTA-6 significantly promoted the two cultivars, proline, chlorophyll content and grassland on the 1st of stem diameter, CC significantly promote the grassland species of stem diameter and 1 prolyl acid.In the water stress experiment, the concentration of a variety of appropriate control regulator to SODM drought and flood the best, but the regulatory effects on alfalfa was no significant difference.