Cloning And Function Preliminary Analysis Of XET Gene From The Cambium Of Anthocephalus Chinensise

A full-length cDNA sequence of AcXET1 gene which copiously expressed in the cambium of Anthocephalus chinensis was cloned by rapid-amplification of cDNA ends and chromosome walking. DNA sequence analysis showed that a 912 bp complete open reading frame which encoded a 303 amino acids protein was in the 1205 bp full cDNA sequence. Blast analyzing results demonstrated that, the homologies of the amino acid sequence of AcXET1 to Actinidia hemsleyan, A. officinalis, Malus×domestica, P. tremula x P. tremuloides, Rosa hybrid cultivar were all above 70%.In order to study the gene function of AcXET1, some bioinformatics methods were used to analize the DNA sequence, and a plant expression vector pBI AcXET1 was constructed by recombinating the open reading frame of AcXET1 and the binary vector pBI121 between the Xba I and Sma I sites. Subsequently AcXET1 gene was introduced into tobacco leaves mediated by Agrobacterium tumerf aciens. Some transgenic resistant plants were proved by PCR and PCR-Sourthen that the AcXET1 gene had integrated into tobacco genome. We found that the transgenetic plants had no diffirence with the wild plant. Then the paraffin section of tobaco stem was made to detect the variations of cell from transgenic plants and control.