AFLP Analysis Of Rust Resistant Gene In Foxtail Millet

Millet rust is one of the most infectious fungus which affects yield of foxtail millet (Setaria italica (L.) Beauv). Therefore, breeding and using herbicide resistant varieties in agriculture production seems to be one of the most effective and economical methods to control Uromyces setariae-italicae (Diet) Yoshino so as to improve yield. According to this, scanning the linkage molecular markers and construct the genetic linkage map for millet rust resistance gene should be very important to isolate and use this resistant gene. It must be of great value for promoting the herbicide resistance breeding in foxtail millet.In this study, the parents, Shilixiang which shows high resitance to foxtail millet rust, Yugu1 which is highly susceptible to foxtail millet rust and their F2 progeny were used as experimental materials for the AFLP analysis of millet rust resistant gene.1. Modified CTAB method was used to extract DNA of foxtail millet, which meets AFLP requirements in high quantity. And then two restriction enzymes MseI and EcoRI were used to deal with the DNA of foxtail millet. AFLP analysis was conducted in the two parents and their F2 progeny. 92 out of the 270 pairs of randomly selected primers amplified the polymorphic bands.2. Three pairs (E-AAC/M-ATA,E-AAC/M-CGT and E-AC/M-CC) of 92 pairs which amplified the polymorphic bands, showed the same discriminating results in replications. The primers E-AAC/M-ATA,E-AAC/M-CGT and E-AC/M-CC produced fragments with the length of 188bp, 212bp and 176bp linked to rust resistant gene Rusi1 with the genetic distance of 9.2 cM, 9.8 cM and 12.4 cM. And then the three AFLP molecular markers were used to construct a genetic linkage map for millet rust resistance gene. The AFLP markers and the genetic linkage map would be facilitated the selection on millet rust resistant gene.3. Modified AFLP molecular marker technique was used in scanning the linkage molecular markers and construct the genetic linkage map for millet rust resistance. According to the already published documents which reported the use of AFLP molecular marker technique in other plants and the features of the DNA of foxtail millet, the factors that eventually affected the results in scanning the linkage molecular markers for millet rust resistance were discussed in details. And finally, a set of modified AFLP molecular marker technique was established, which is of great use in scanning the linkage molecular markers and construct the genetic linkage map for millet rust resistance.