Study Of Cold Resistance Of Floral Organ Of Kernel Apricot Superior Strain

The test take the floral organs of anti-frost Kernel Apricot(Prunus armeniaca×sibirica) superior strains as test materials, studied cold hardiness of 36 superior strains of kernel apricot to frost hazard,and used the pistil of Kernel Apricot of selected superior strains of the most cold stress as the specimens,explore a set of two dimensional electropHoresis technology that applied to separate the whole-cell proteins of the pistil of Kernel Apricot, for further research mechanism of cold hardiness of Kernel apricot, filtrate cold resistant varieties and further lay the foundation for breeding. The results are as follows:1. In the 28 (Prunus armeniaca×sibirica'youyi') 'youyi' superior strains to cold stress, average value of the subordinate function of the supercooling point,the electrolyte leakage,superoxide dismutase activity of the petal, stamen and pistil of'25'is 0.3334,0.3334,0.3027,the lagest one.Rank order of The superior strains about different floral organs is different , and not only comprehensive ranking of ' 25', but also the ranking of different floral organs is first.2 In the 8 'longwangmao' (Prunus armeniaca×sibirica'longwangmao') superior strains to cold stress, average value of the subordinate function of the supercooling point,the electrolyte leakage,superoxide dismutase activity of the petal, stamen and pistil of '31' is 0.3334,0.3201,0.3334,the lagest one. Rank order of the superior strains about different floral organs is different , and not only comprehensive ranking of ' 31', but also the ranking of different floral organs is first.3. The freezing rate of ' 25'and' 31'in the field were lighter than the contrast . The freezing rate of petals and pistils of' 25'is lower than the contrast were 9.4%, 7.8%; the freezing rate of petals and pistils of ' 31' is lower than the contrast were 12.5%, 4.9%; there are significant differences between superior strains and contrast (P <0.05). Fruit set of superior strains after freeze injury was better than contrast, and the difference is significant (P <0.05). From the freezing rate of petals pistil of view, difference of the freezing rate of '25' and ' 31' were not significantly different; from the fruit setting rate of view after freeze injury, the fruit setting rate of '25'was taller than ' 31', the difference was significant (P <0.05).4. Supercooling point temperature of superior strains of various floral organs is lower than the contrast, the difference was significant (P <0.05), ' 25' reached significant level (P <0.01), supercooling point temperature of petal, stamen, pistil of '25 'is lower than the same varieties, is 0.9℃, the supercooling points temperature of petals, stamen, pistil of ' 31 'is lower than the same varieties ,were 0.5℃, 0.7℃, 0.7℃. supercooling point temperature of petals, stamen, pistil of '25' is lower than ' 31 ',is 0.7℃, 0.4℃, 0.5℃, there are differences at the P <0.01 level to the two of petals, stamens,and pistil is different at the P <0.05 level .5. With the temperature declining, electrolyte leakage, MDA content of different varieties of flower organs is continuance rising , soluble protein and SOD activity was first increased and then decreased.The electrolyte leakage,MDA content of superior strains of flower organs is lower than contrast under the different temperature。electrolyte leakage, MDA content of two superior strains of floral organs showed ' 25' <'" 31'. lethal temperatures of superior strains were lower than the contrastl. Results is accordance with the results of electrolyte leakage.SOD activity is different between the sperior strains and the contrast,maximum SOD valueof petal, stamen , pistil of '25' appeared at -6℃, -3℃, -3℃,maximum SOD activity of petal, stamen , pistil of contrast appeared at -5℃, -3℃, -3℃.In addition to the SOD value of the pistil with -6℃, under the different low temperature stress , the SOD value of floral organs of '25' were higher than contrast.maximum SOD valueof petal, stamen , pistil of '31' appeared at -5℃, -3℃, -3℃,maximum SOD activity of petal, stamen , pistil of contrast appeared at -4℃, -3℃, -3℃.In addition to the SOD value of the petalwith -4℃,and the stamen and pistil with-7℃, under the different low temperature stress ,the SOD value of floral organs of '31' were higher than contrast.SOD activity of diffrent superior strains floral organs is diffrent, showing '25'> '31'.Soluble protein content of petals of 'longwangmao' is maximum in -3℃, soluble protein content of the others reached maximum at -4℃. under the low temperature stress, soluble protein content of floral organ of superior strains were higher than contrast. soluble protein content of stamen, pistil of ' 31'from -6℃to -7℃is digger than '25', the rest showed ' 25'> '31'.3. Established a set of two dimensional electropHoresis technology that fit proteomics of the pistil of Kernel Apricot。Namely,using combined the TCA / acetone and pHenol extraction method to distill the pistil proteins,dissolve protein powder with lysis 2h at normal temperature shock method, followed by bradford method determine protein concentration, on this basis, the content of sample 100ug, the sample volume of 370ul. Subsequently, first,isoelectric focusing using pH 5-8,17 cm linear IPG strips for protein separation, after isoelectric focusing using balance bufferⅠ,Ⅱthat each contain DTT and iodoacetamide balance strips 15min. The second ,the SDS-PAGE, using 12% gel concentration , separate protein. The gel stained by silver contained, in the end,the gel map with preferably distinguishability and reproducibility was obtained.