Associations Of Polymorphisms Of CSN1S1 & CSN1S2 Genes With Economic Traits In Dairy Goats

To obtain molecular genetic marker related to economic traits in dairy goats, the genetic variations of 11 loci from CSN1S1(exon 2, 9, 12, 17, 19 and intron 14) and CSN1S2 (exon 2, 3, 9, 11, 16) genes and their associations with body size (withers height, body length and heart girth), milk yield, and milk composition (fat, protein, lactose, total solids, solids-not-fat and density) traits in 708 samples of Xinong Sannen (XS, n=440) and Guanzhong (GZ, n=268) breeds were analyzed using bioinformatics, DNA sequencing, PCR-SSCP,PCR-RFLP and AS-PCR techniques. The important results were in the followings:1. Genetic variation of CSN1S1 gene and their assosiations with economic traits in dairy goatsThe varations of six loci (P1~P6) in CSN1S1 gene were studied in populations of XS and GZ. In these six loci, one nucleotide (C) and seven nuleotides (TTATCA) deletions were detected in P2 and P6 locus respectively, as well as P3 locus, the same mutation was found as the previously published result, while no polymorphisms were detected in P1, P4 and P5 locus.â‘ There were three genotypes in the P2 locus of CSN1S1 gene: AA,AB and BB. The frequences of A/B allele were 0.198/0.802 and 0.361/0.639 in XS and GZ breeds respectivelyAt the P2 locus, both of the populations were moderate polymorphic (0.25