Genetic Diversity Of Different Geographic Populations Of Oedaleus Asiaticus In Inner Mongolia

The genetic divergence of natural populations of Oedaleus asiaticus Bienko was studied by Random Amplified Polymorphic DNA (RAPD). Genomic DNA of 90 individuals were amplified by eight random primers. A total of 78 clear and reproducible bands were generated, 62 of which were polymorphic, and Nei's index and a genetic distance matrix were calculated. The gene differentiation coefficient between populations estimated by Nei' s index was 0.2343, indicating that 23.43% of the variation occurred between populations. The gene differentiation coefficient within populations was 0.7657, indicating that 76.57% of all variation was within populations. The gene differentiation coefficient within populations was evidently higher than that between populations. A dendrogram based on Nei's genetic distance of RAPD markers was constructed using the NJ method. The results indicate that genetic divergence in populations of O. asiaticus corresponds to both geographic and genetic distance.28 individuals of Oedaleus asiaticus Bienko distributed in 7 different geographical regions of Inner Mongolia have been measured by the mitochondrial DNA (mtDNA) ND1 and 16S rRNA partial sequences,and their sequences identity were compared and their nucleotide composition were calculated, and then, phylogenetic trees were built by means of UPGMA and NJ with Myrmeleotettix palpalis Zub. and Angaracris barabensis Pallas as the control. The sequence composition of the Asian Oedaleus ND1 gene was 303bp from 12248-12595 (347 bp), which had been edited and cut. Meanwhile this study showed that the average content of A and T was 76.6% and 22 variable sites was detected, about 7.26% of nucleotide, the majority of mutations occur in the third loci(accounting for 68.18%). For each amino acid codon of view, the third points was rich in A and T, as much as 90.3%. The NJ phylogenetic tree showed that there were some differences in ND1 gene of different haplotypes, and there was no direct correspondence between the formation of clusters of different kinds of relationship with the geographic distribution of its branches. But overall, the relationships of this cluster were parallel-type distribution, and there were no significant differences between regions. A sequence of 267bp length was obtained by editing the sequences 12887-13176 (289 bp) of 16S rRNA sequence, and the average content of A and T was 70.3%. Meanwhile 20 variable sites were detected, which accounted for 7.49% of total nucleotides. The majority of mutations occurred in the third loci (50.0%). In respect of each amino acid, the content of A and T was rich in the third point with about 75.1%. UPGMA phylogenetic tree and NJ phylogenetic tree demonstrated the same result that mutations occurred mainly in the sites of Tongliao, Chifeng and Hulunbeier, and the other individual variable sites were less likely to be more conservative 16S rRNA gene.