Indentification And Population Differentiation Of Pathogen Caused Grape Anthracnose In China

The grape anthracnose has become an important grape production disease in china.This study investigated the grape anthracnose of China, and used the biological characteristics and the AFLP markers for the grape anthracnose strains, the results were as follows:1 Though the investigating on Beijing ,Shandong,Henan,Heibei,the grape anthracnose occurs accordingly. The max incidence rate is up to 55%.2 A total of 80 isolates collected from grape anthracnose. The morphological and rDNA ITS region sequence showed that the isolates were Colletotrichum gloeosporioides (Penz.) Sacc.3 Based on the significant difference about colony morphology, sporulation, growth rate and virulence, we made all the strains of C. gloeosporioides strains into three groups, named groupⅠ,Ⅱ,Ⅲ.4 The genetic diversity and relationship of 3 groups'strains of grape were evaluated using AFLP in this study.The results showed that a total of 1059 informative AFLP fragments were generated with 10 EcoRΙ-MseΙprimer combinations,of which 489 ones were polymorphic and the polymorphism rate was 32.14%. And the result showed there are differences among three pathogenic group strains at the molecular level:Randomly selected 20 strains of C. gloeosporioides from three different pathogenic groups to verify the AFLP primers. Amplification results was 17 strains of anthracnose consistent with morphological classification. Biology and morphology of two delineation AFLP marker similarity of 85%.